Effect of in vivo coal dust exposure on arachidonic acid metabolism in the rat alveolar macrophage

Charles F. Stanley, Nadia El-Ayouby, Laurence M. Demers, C. Kuhn Douglas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Oxygenated metabolites of arachidonic acid (AA) are produced by the alveolar macro-phage (AM) and have been shown to mediate inflammatory reactions. We therefore assessed the production of eicosanoids by AM harvested from the lungs of rats exposed to a bituminous coal dust for 2 wk in an inhalation chamber in order to determine if AA metabolism was altered in a manner that may promote an inflammatory response in the lung. Exposure to coal dust resulted in a 66% increase in the number of AM harvested, an increase in thromboxane A2 (TxA2) and leukotriene B4 (LTBJ production to 222% and 181% of control values, respectively, and a decrease in prostaglandin E2 (PCE2) production to 62% of control values. In AM harvested from rats allowed to breath clean air for 2 wk following coal dust exposure, PCE2 production returned to control levels but TxA2 and LTB4 production remained elevated. The TxA2 synthesis inhibitor UK 38, 485 reduced TxA2 production in dust-exposed AM both immediately and 2 wk following exposure. Thus, exposure of rats to coal dust significantly alters the metabolism of AA in AM, with potentially important aspects of AA metabolism remaining altered even after a 2-wk recovery period. Based on the established role of eicosanoids in inflammatory and fibrotic processes, these results suggest that the alteration of AM eicosanoid production as a result of the inhalation of coal mine dust may be an important factor in the pathophysiology of coal workers’ pneumoconiosis.

Original languageEnglish (US)
Pages (from-to)157-160
Number of pages4
JournalJournal of Toxicology and Environmental Health
Volume29
Issue number2
DOIs
StatePublished - Feb 1 1990

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Coal dust
Bacteriophages
Coal
Alveolar Macrophages
Dust
Arachidonic Acid
Metabolism
Macros
Rats
Thromboxane A2
Acids
Eicosanoids
Leukotriene B4
Inhalation
Mine dust
Anthracosis
Lung
Bituminous coal
Level control
Metabolites

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pollution

Cite this

Stanley, Charles F. ; El-Ayouby, Nadia ; Demers, Laurence M. ; Douglas, C. Kuhn. / Effect of in vivo coal dust exposure on arachidonic acid metabolism in the rat alveolar macrophage. In: Journal of Toxicology and Environmental Health. 1990 ; Vol. 29, No. 2. pp. 157-160.
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Effect of in vivo coal dust exposure on arachidonic acid metabolism in the rat alveolar macrophage. / Stanley, Charles F.; El-Ayouby, Nadia; Demers, Laurence M.; Douglas, C. Kuhn.

In: Journal of Toxicology and Environmental Health, Vol. 29, No. 2, 01.02.1990, p. 157-160.

Research output: Contribution to journalArticle

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