Effect of inhibitors of protein synthesis on rat liver spermidine N1-acetyltransferase

Matsui Isao Matsui, Anthony Pegg

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34 Scopus citations

Abstract

The increase in spermidine N1-acetyltransferase activity in rat liver produced by carbon tetrachloride was completely prevented by simultaneous treatment with inhibitors of protein and nucleic acid synthesis suggesting that the increase results from the synthesis of new protein rather than the release of the enzyme from a cryptic inactive form. Treatment with cycloheximide 2 h after carbon tetrachloride also completely blocked the rise in spermidine N1-acetyltransferase seen 4 h later. Such treatment completely prevented the fall in spermidine and rise in putrescine in the liver 6 h after carbon tetrachloride confirming the importance of the induction of spermidine N1-acetyltransferase in the conversion of spermidine into putrescine. When cycloheximide was administered to rats in which spermidine N1-acetyltransferase activity had been stimulated by prior treatment with carbon tetrachloride or thioacetamide, the activity was lost rapidly showing that the enzyme protein has a rapid rate of turnover. The half-life for the enzyme in thioacetamide-treated rats was 40 min, whereas the half-life for ornithine decarboxylase (which is well known to turn over very rapidly) was 27 min. In carbon tetrachloride-treated rats the rate of protein degradation was reduced and the half-life of spermidine N1-acetyltransferase was 155 min and that for ornithine decarboxylase was 65 min. It appears that three of the enzymes involved in the synthesis and interconversion of putrescine and spermidine namely, ornithine decarboxylase, S-adenosylmethionine decarboxylase and spermidine N1-acetyltransferase have rapid rates of turnover and that polyamine levels are regulated by changes in the amount of these enzymes.

Original languageEnglish (US)
Pages (from-to)373-378
Number of pages6
JournalBBA - General Subjects
Volume675
Issue number3-4
DOIs
StatePublished - Jul 17 1981

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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