Background: Several aspects of the inflammatory response to a single insult, i.e., exposure to 2 ppm of ozone (O3) for 3 h or 6 h, are less pronounced in surfactant protein A deficient (SP-A -/-) mice (KO) than in wild type mice (WT). It was hypothesized that a mild insult, specifically low doses of lipopolysaccharide (LPS), would adversely affect host defense and differentially potentiate O3-induced injury in WT and KO mice. Methods: WT and KO mice were treated with different doses of LPS or LPS (2 ng) + O3 (2 ppm) or filtered air (FA) for 3 h, then sacrificed 4 h following exposure (O3, FA) or 20 h after LPS treatment alone. Several endpoints of inflammation were measured in bronchoalveolar lavage (BAL). Results: 1) At 20 h after LPS treatment alone, both WT and KO mice exhibited signs of inflammation, but with differences in the macrophage inflammatory protein 2 (MIP-2) response pattern, total cells (at 0.5 ng LPS) and basal levels of oxidized protein and phospholipids; 2) After LPS + O3, KO compared to WT showed decrease in polymorphonuclear leukocytes (PMNs) and MIP-2 and increase in phospholipids, and after LPS + FA an increase in total cells; 3) WT after LPS + FA showed an increase in SP-A with no further increase after LPS + O3, and an increase in oxidized SP-A dimer following O3 or LPS + O3. Conclusions: LPS treatment has negative effects on inflammation endpoints in mouse BAL long after exposure and renders KO mice less capable of responding to a second insult. LPS and O3 affect SP-A, quantitatively and qualitatively, respectively.
|Original language||English (US)|
|Number of pages||13|
|State||Published - 2009|
All Science Journal Classification (ASJC) codes
- Pulmonary and Respiratory Medicine