Effect of Media Composition on Growth, Plasmid Stability and Ovine Interferonλ Production in Saccharomyces cerevisiae

Vijay Chiruvolu, Jayne M. Stratton, Troy Ott, Fuller W. Bazer, Michael M. Meagher

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Recombinant protein production is often influenced by the stability of plasmid carrying the cloned gene and the final biomass yield. Ovine (sheep) interferon-λ (oIFNλ) has been expressed in Saccharomyces cerevisiae under the regulation of a hybrid alcohol dehydrogenase 2/glyceraldehyde-3-phosphate dehydrogenase promoter. The influence of media composition on growth rate, biomass yield, plasmid stability and oIFNλ were studied. In a selective medium, with leucine and uracil selection, the plasmid stability was good but the yeast failed to grow to high cell densities. In 60-l fermentations, oIFNλ yields of 49 mg/l were achieved for complex media and about 3.4 mg/l for casamino acids with uracil selection based on bioassay. A fraction of the protein was insoluble and was solubilized by 6 M guanidine hydrochloride.

Original languageEnglish (US)
Pages (from-to)565-569
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume82
Issue number6
DOIs
StatePublished - Jan 1 1996

Fingerprint

Interferons
Yeast
Saccharomyces cerevisiae
Sheep
Plasmids
Uracil
Biomass
Growth
Chemical analysis
Recombinant proteins
Glyceraldehyde-3-Phosphate Dehydrogenases
Bioassay
Alcohol Dehydrogenase
Guanidine
Recombinant Proteins
Leucine
Biological Assay
Fermentation
Phosphates
Alcohols

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Chiruvolu, Vijay ; Stratton, Jayne M. ; Ott, Troy ; Bazer, Fuller W. ; Meagher, Michael M. / Effect of Media Composition on Growth, Plasmid Stability and Ovine Interferonλ Production in Saccharomyces cerevisiae. In: Journal of Fermentation and Bioengineering. 1996 ; Vol. 82, No. 6. pp. 565-569.
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Effect of Media Composition on Growth, Plasmid Stability and Ovine Interferonλ Production in Saccharomyces cerevisiae. / Chiruvolu, Vijay; Stratton, Jayne M.; Ott, Troy; Bazer, Fuller W.; Meagher, Michael M.

In: Journal of Fermentation and Bioengineering, Vol. 82, No. 6, 01.01.1996, p. 565-569.

Research output: Contribution to journalArticle

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