Effect of Media Composition on Growth, Plasmid Stability and Ovine Interferonλ Production in Saccharomyces cerevisiae

Vijay Chiruvolu, Jayne M. Stratton, Troy Ott, Fuller W. Bazer, Michael M. Meagher

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Recombinant protein production is often influenced by the stability of plasmid carrying the cloned gene and the final biomass yield. Ovine (sheep) interferon-λ (oIFNλ) has been expressed in Saccharomyces cerevisiae under the regulation of a hybrid alcohol dehydrogenase 2/glyceraldehyde-3-phosphate dehydrogenase promoter. The influence of media composition on growth rate, biomass yield, plasmid stability and oIFNλ were studied. In a selective medium, with leucine and uracil selection, the plasmid stability was good but the yeast failed to grow to high cell densities. In 60-l fermentations, oIFNλ yields of 49 mg/l were achieved for complex media and about 3.4 mg/l for casamino acids with uracil selection based on bioassay. A fraction of the protein was insoluble and was solubilized by 6 M guanidine hydrochloride.

Original languageEnglish (US)
Pages (from-to)565-569
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume82
Issue number6
DOIs
StatePublished - Jan 1 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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