Effect of Media Composition on Growth, Plasmid Stability and Ovine Interferonλ Production in Saccharomyces cerevisiae

Vijay Chiruvolu, Jayne M. Stratton, Troy L. Ott, Fuller W. Bazer, Michael M. Meagher

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Recombinant protein production is often influenced by the stability of plasmid carrying the cloned gene and the final biomass yield. Ovine (sheep) interferon-λ (oIFNλ) has been expressed in Saccharomyces cerevisiae under the regulation of a hybrid alcohol dehydrogenase 2/glyceraldehyde-3-phosphate dehydrogenase promoter. The influence of media composition on growth rate, biomass yield, plasmid stability and oIFNλ were studied. In a selective medium, with leucine and uracil selection, the plasmid stability was good but the yeast failed to grow to high cell densities. In 60-l fermentations, oIFNλ yields of 49 mg/l were achieved for complex media and about 3.4 mg/l for casamino acids with uracil selection based on bioassay. A fraction of the protein was insoluble and was solubilized by 6 M guanidine hydrochloride.

Original languageEnglish (US)
Pages (from-to)565-569
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume82
Issue number6
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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