Effect of natural organic matter on zinc inhibition of hematite bioreduction by Shewanella putrefaciens CN32

James J. Stone, Richard A. Royer, Brian A. Dempsey, William D. Burgos

Research output: Contribution to journalArticle

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Abstract

The effect of zinc on the biological reduction of hematite (α-Fe 2O3) by the dissimilatory metal-reducing bacterium (DMRB) Shewanella putrefaciens CN32 was studied in the presence of four natural organic materials (NOMs). Experiments were performed under non-growth conditions with H2 as the electron donor and zinc inhibition was quantified as the decrease in the 5 d extent of hematite bioreduction as compared to no-zinc controls. Every NOM was shown to significantly increase zinc inhibition during hematite bioreduction. NOMs were shown to alter the distribution of both biogenic Fe(II) and Zn(II) between partitioned (hematite and cell surfaces) and solution phases. To further evaluate the mechanism(s) of NOM-promoted zinc inhibition, similar bioreduction experiments were conducted with nitrate as a soluble electron acceptor, and hematite bioreduction experiments were conducted with manganese which was essentially non-inhibitory in the absence of NOM. The results suggest that Me(II)-NOM complexes may be specifically inhibitory during solid-phase bioreduction via interference of DMRB attachment to hematite through the formation of ternary Me(II)-NOM-hematite complexes.

Original languageEnglish (US)
Pages (from-to)5284-5290
Number of pages7
JournalEnvironmental Science and Technology
Volume41
Issue number15
DOIs
StatePublished - Aug 1 2007

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Biological materials
hematite
Zinc
zinc
organic matter
Bacteria
Metals
electron
bacterium
Electrons
experiment
Experiments
metal
Manganese
effect
material
ferric oxide
Nitrates
manganese
nitrate

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

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title = "Effect of natural organic matter on zinc inhibition of hematite bioreduction by Shewanella putrefaciens CN32",
abstract = "The effect of zinc on the biological reduction of hematite (α-Fe 2O3) by the dissimilatory metal-reducing bacterium (DMRB) Shewanella putrefaciens CN32 was studied in the presence of four natural organic materials (NOMs). Experiments were performed under non-growth conditions with H2 as the electron donor and zinc inhibition was quantified as the decrease in the 5 d extent of hematite bioreduction as compared to no-zinc controls. Every NOM was shown to significantly increase zinc inhibition during hematite bioreduction. NOMs were shown to alter the distribution of both biogenic Fe(II) and Zn(II) between partitioned (hematite and cell surfaces) and solution phases. To further evaluate the mechanism(s) of NOM-promoted zinc inhibition, similar bioreduction experiments were conducted with nitrate as a soluble electron acceptor, and hematite bioreduction experiments were conducted with manganese which was essentially non-inhibitory in the absence of NOM. The results suggest that Me(II)-NOM complexes may be specifically inhibitory during solid-phase bioreduction via interference of DMRB attachment to hematite through the formation of ternary Me(II)-NOM-hematite complexes.",
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Effect of natural organic matter on zinc inhibition of hematite bioreduction by Shewanella putrefaciens CN32. / Stone, James J.; Royer, Richard A.; Dempsey, Brian A.; Burgos, William D.

In: Environmental Science and Technology, Vol. 41, No. 15, 01.08.2007, p. 5284-5290.

Research output: Contribution to journalArticle

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AB - The effect of zinc on the biological reduction of hematite (α-Fe 2O3) by the dissimilatory metal-reducing bacterium (DMRB) Shewanella putrefaciens CN32 was studied in the presence of four natural organic materials (NOMs). Experiments were performed under non-growth conditions with H2 as the electron donor and zinc inhibition was quantified as the decrease in the 5 d extent of hematite bioreduction as compared to no-zinc controls. Every NOM was shown to significantly increase zinc inhibition during hematite bioreduction. NOMs were shown to alter the distribution of both biogenic Fe(II) and Zn(II) between partitioned (hematite and cell surfaces) and solution phases. To further evaluate the mechanism(s) of NOM-promoted zinc inhibition, similar bioreduction experiments were conducted with nitrate as a soluble electron acceptor, and hematite bioreduction experiments were conducted with manganese which was essentially non-inhibitory in the absence of NOM. The results suggest that Me(II)-NOM complexes may be specifically inhibitory during solid-phase bioreduction via interference of DMRB attachment to hematite through the formation of ternary Me(II)-NOM-hematite complexes.

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