Effect of repeated in vitro transfer of Pasteurella haemolytica A1 on encapsulation, leukotoxin production, and virulence

M. J. Gentry, A. W. Confer, Rebecca Craven

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Abstract

Pasteurella haemolytica serotype 1 was transferred daily for 128 serial passages on both unsupplemented brain heart infusion agar and the same basal medium supplemented with bovine blood, horse serum, and yeast extract. Repeatedly transferred cultures were shown to retain the ability to produce both capsular material and leukotoxin. Furthermore, intact organisms were found to be as toxic for bovine leukocytes and as virulent for mice as unpassaged cultures. These results indicate that the precaution of using only freshly isolated cultures in the study of this organism may not be necessary.

Original languageEnglish (US)
Pages (from-to)142-145
Number of pages4
JournalJournal of Clinical Microbiology
Volume25
Issue number1
Publication statusPublished - 1987

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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