Effect of rhamnolipids on permeability across Caco-2 cell monolayers

Charity J. Wallace, Scott H. Medina, Mohamed E.H. ElSayed

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: This report describes the effect of rhamnolipids (RLs), an amphiphilic biosurfactant produced by the bacterium Pseudomonas aeruginosa, on the integrity and permeability across Caco-2 cell monolayers. Methods: We measured the trans-epithelial electrical resistance (TEER) and permeability of [14C]mannitol across Caco-2 cell monolayers upon incubation with 0.01-5.0% v/v RLs as a function of incubation time (30, 60, 90, and 120 min). We also studied the recovery of RL-treated Caco-2 cell monolayers upon incubation with Kaempferol, which is a natural flavonoid that promotes the assembly of the tight junctions. Results: TEER of Caco-2 cell monolayers incubated with 0.01-5.0% v/v RLs solution dropped to 80-28% of that of untreated cells. Decline in TEER was associated with an increase in [14C]mannitol permeability as a function of RLs concentration and incubation time with Caco-2 cells. Incubation of RLs-treated Caco-2 cell monolayers with normal culture medium for 48 h did not restore barrier integrity. Whereas, incubation of a RLs-treated Caco-2 cells with culture medium containing Kaempferol for 24 h restored barrier function indicated by the higher TEER and lower [ 14C]mannitol permeability values. Conclusions: These results show the ability of RLs to modulate the integrity and permeability of Caco-2 cell monolayers in a concentration- and time-dependent fashion, which suggest their potential to function as a non-toxic permeation enhancer.

Original languageEnglish (US)
Pages (from-to)887-894
Number of pages8
JournalPharmaceutical Research
Volume31
Issue number4
DOIs
StatePublished - Apr 2014

Fingerprint

Caco-2 Cells
Monolayers
Permeability
Acoustic impedance
Electric Impedance
Mannitol
Culture Media
rhamnolipid
Tight Junctions
Flavonoids
Permeation
Pseudomonas aeruginosa
Bacteria
Cell Culture Techniques
Recovery

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

Cite this

Wallace, Charity J. ; Medina, Scott H. ; ElSayed, Mohamed E.H. / Effect of rhamnolipids on permeability across Caco-2 cell monolayers. In: Pharmaceutical Research. 2014 ; Vol. 31, No. 4. pp. 887-894.
@article{098b595be8874cb9b11dc196a717828a,
title = "Effect of rhamnolipids on permeability across Caco-2 cell monolayers",
abstract = "Purpose: This report describes the effect of rhamnolipids (RLs), an amphiphilic biosurfactant produced by the bacterium Pseudomonas aeruginosa, on the integrity and permeability across Caco-2 cell monolayers. Methods: We measured the trans-epithelial electrical resistance (TEER) and permeability of [14C]mannitol across Caco-2 cell monolayers upon incubation with 0.01-5.0{\%} v/v RLs as a function of incubation time (30, 60, 90, and 120 min). We also studied the recovery of RL-treated Caco-2 cell monolayers upon incubation with Kaempferol, which is a natural flavonoid that promotes the assembly of the tight junctions. Results: TEER of Caco-2 cell monolayers incubated with 0.01-5.0{\%} v/v RLs solution dropped to 80-28{\%} of that of untreated cells. Decline in TEER was associated with an increase in [14C]mannitol permeability as a function of RLs concentration and incubation time with Caco-2 cells. Incubation of RLs-treated Caco-2 cell monolayers with normal culture medium for 48 h did not restore barrier integrity. Whereas, incubation of a RLs-treated Caco-2 cells with culture medium containing Kaempferol for 24 h restored barrier function indicated by the higher TEER and lower [ 14C]mannitol permeability values. Conclusions: These results show the ability of RLs to modulate the integrity and permeability of Caco-2 cell monolayers in a concentration- and time-dependent fashion, which suggest their potential to function as a non-toxic permeation enhancer.",
author = "Wallace, {Charity J.} and Medina, {Scott H.} and ElSayed, {Mohamed E.H.}",
year = "2014",
month = "4",
doi = "10.1007/s11095-013-1210-5",
language = "English (US)",
volume = "31",
pages = "887--894",
journal = "Pharmaceutical Research",
issn = "0724-8741",
publisher = "Springer New York",
number = "4",

}

Effect of rhamnolipids on permeability across Caco-2 cell monolayers. / Wallace, Charity J.; Medina, Scott H.; ElSayed, Mohamed E.H.

In: Pharmaceutical Research, Vol. 31, No. 4, 04.2014, p. 887-894.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of rhamnolipids on permeability across Caco-2 cell monolayers

AU - Wallace, Charity J.

AU - Medina, Scott H.

AU - ElSayed, Mohamed E.H.

PY - 2014/4

Y1 - 2014/4

N2 - Purpose: This report describes the effect of rhamnolipids (RLs), an amphiphilic biosurfactant produced by the bacterium Pseudomonas aeruginosa, on the integrity and permeability across Caco-2 cell monolayers. Methods: We measured the trans-epithelial electrical resistance (TEER) and permeability of [14C]mannitol across Caco-2 cell monolayers upon incubation with 0.01-5.0% v/v RLs as a function of incubation time (30, 60, 90, and 120 min). We also studied the recovery of RL-treated Caco-2 cell monolayers upon incubation with Kaempferol, which is a natural flavonoid that promotes the assembly of the tight junctions. Results: TEER of Caco-2 cell monolayers incubated with 0.01-5.0% v/v RLs solution dropped to 80-28% of that of untreated cells. Decline in TEER was associated with an increase in [14C]mannitol permeability as a function of RLs concentration and incubation time with Caco-2 cells. Incubation of RLs-treated Caco-2 cell monolayers with normal culture medium for 48 h did not restore barrier integrity. Whereas, incubation of a RLs-treated Caco-2 cells with culture medium containing Kaempferol for 24 h restored barrier function indicated by the higher TEER and lower [ 14C]mannitol permeability values. Conclusions: These results show the ability of RLs to modulate the integrity and permeability of Caco-2 cell monolayers in a concentration- and time-dependent fashion, which suggest their potential to function as a non-toxic permeation enhancer.

AB - Purpose: This report describes the effect of rhamnolipids (RLs), an amphiphilic biosurfactant produced by the bacterium Pseudomonas aeruginosa, on the integrity and permeability across Caco-2 cell monolayers. Methods: We measured the trans-epithelial electrical resistance (TEER) and permeability of [14C]mannitol across Caco-2 cell monolayers upon incubation with 0.01-5.0% v/v RLs as a function of incubation time (30, 60, 90, and 120 min). We also studied the recovery of RL-treated Caco-2 cell monolayers upon incubation with Kaempferol, which is a natural flavonoid that promotes the assembly of the tight junctions. Results: TEER of Caco-2 cell monolayers incubated with 0.01-5.0% v/v RLs solution dropped to 80-28% of that of untreated cells. Decline in TEER was associated with an increase in [14C]mannitol permeability as a function of RLs concentration and incubation time with Caco-2 cells. Incubation of RLs-treated Caco-2 cell monolayers with normal culture medium for 48 h did not restore barrier integrity. Whereas, incubation of a RLs-treated Caco-2 cells with culture medium containing Kaempferol for 24 h restored barrier function indicated by the higher TEER and lower [ 14C]mannitol permeability values. Conclusions: These results show the ability of RLs to modulate the integrity and permeability of Caco-2 cell monolayers in a concentration- and time-dependent fashion, which suggest their potential to function as a non-toxic permeation enhancer.

UR - http://www.scopus.com/inward/record.url?scp=84897011523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897011523&partnerID=8YFLogxK

U2 - 10.1007/s11095-013-1210-5

DO - 10.1007/s11095-013-1210-5

M3 - Article

C2 - 24065598

AN - SCOPUS:84897011523

VL - 31

SP - 887

EP - 894

JO - Pharmaceutical Research

JF - Pharmaceutical Research

SN - 0724-8741

IS - 4

ER -