Effect of rotavirus strain on the murine model of biliary atresia

Steven Allen, Mubeen Jafri, Bryan Donnelly, Monica McNeal, David Witte, Jorge Bezerra, Richard Ward, Gregory M. Tiao

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Biliary atresia is a devastating disorder of the newborn in which afflicted infants develop inflammation and fibrosis of the extrahepatic biliary tract, resulting in cirrhosis and end-stage liver disease. Infection with a virus is thought to be a contributing factor in the etiology of biliary atresia. In the murine model of biliary atresia, perinatal exposure to rhesus rotavirus (RRV) results in biliary epithelial cell infection causing bile duct obstruction. The purpose of this study was to determine if tropism for the biliary epithelial cell was unique to RRV. Newborn mice underwent intraperitoneal injection with five strains of rotavirus: RRV (simian), SA11-FM (simian/bovine), SA11-SM (simian), EDIM (murine), and Wa (human). RRV and SA11-FM caused clinical manifestations of bile duct obstruction and high mortality. SA11-SM caused clinical signs of hepatobiliary injury but the mortality was markedly reduced. EDIM and Wa caused no sign of hepatobiliary disease. The systemic and temporal distribution of viral protein and live virus varied according to the injected strain. Immunohistochemistry revealed that RRV and SA11-FM targeted the biliary epithelial cells. In contrast, SA11-SM was found in the liver but in not in the biliary epithelium. These results indicate that strain-specific characteristics dictate tropism for cells of hepatobiliary origin which in turn impact the ability to induce the murine model of biliary atresia.

Original languageEnglish (US)
Pages (from-to)1671-1679
Number of pages9
JournalJournal of virology
Volume81
Issue number4
DOIs
StatePublished - Feb 1 2007

Fingerprint

Biliary Atresia
Rotavirus
abnormal development
animal models
cholestasis
tropisms
epithelial cells
Tropism
Epithelial Cells
Cholestasis
neonates
Fibrosis
biliary tract
Viruses
viruses
End Stage Liver Disease
Mortality
viral proteins
mice
Biliary Tract

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Allen, S., Jafri, M., Donnelly, B., McNeal, M., Witte, D., Bezerra, J., ... Tiao, G. M. (2007). Effect of rotavirus strain on the murine model of biliary atresia. Journal of virology, 81(4), 1671-1679. https://doi.org/10.1128/JVI.02094-06
Allen, Steven ; Jafri, Mubeen ; Donnelly, Bryan ; McNeal, Monica ; Witte, David ; Bezerra, Jorge ; Ward, Richard ; Tiao, Gregory M. / Effect of rotavirus strain on the murine model of biliary atresia. In: Journal of virology. 2007 ; Vol. 81, No. 4. pp. 1671-1679.
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Allen, S, Jafri, M, Donnelly, B, McNeal, M, Witte, D, Bezerra, J, Ward, R & Tiao, GM 2007, 'Effect of rotavirus strain on the murine model of biliary atresia', Journal of virology, vol. 81, no. 4, pp. 1671-1679. https://doi.org/10.1128/JVI.02094-06

Effect of rotavirus strain on the murine model of biliary atresia. / Allen, Steven; Jafri, Mubeen; Donnelly, Bryan; McNeal, Monica; Witte, David; Bezerra, Jorge; Ward, Richard; Tiao, Gregory M.

In: Journal of virology, Vol. 81, No. 4, 01.02.2007, p. 1671-1679.

Research output: Contribution to journalArticle

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AU - Allen, Steven

AU - Jafri, Mubeen

AU - Donnelly, Bryan

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AU - Ward, Richard

AU - Tiao, Gregory M.

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Allen S, Jafri M, Donnelly B, McNeal M, Witte D, Bezerra J et al. Effect of rotavirus strain on the murine model of biliary atresia. Journal of virology. 2007 Feb 1;81(4):1671-1679. https://doi.org/10.1128/JVI.02094-06