Effect of tamoxifen on fatty degeneration and atrophy of rotator cuff muscles in chronic rotator cuff tear: An animal model study

Edward Cho, Yue Zhang, Anne Pruznak, Hyun-Min Mike Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Fatty degeneration of the rotator cuff muscles is an irreversible change resulting from chronic rotator cuff tear and is associated with poor clinical outcomes following rotator cuff repair. We evaluated the effect of Tamoxifen, a competitive estrogen receptor inhibitor, on fatty degeneration using a mouse model for chronic rotator cuff tear. Sixteen adult mice were divided into two diet groups (Tamoxifen vs. Regular) and subjected to surgical creation of a large rotator cuff tear and suprascapular nerve transection in their left shoulder with the right shoulder serving as a control. The rotator cuff muscles were harvested at 16 weeks and subjected to histology and RT-PCR for adipogenic and myogenic markers. Histology showed substantially decreased atrophy and endomysial inflammation in Tamoxifen group, but no significant differences in the amount of intramuscular adipocytes and lipid droplets compared to the Regular group. With RT-PCR, the operated shoulders showed significant upregulation of myogenin and PPAR-γ, and downregulation of myostatin compared to the nonsurgical shoulder. No significant differences of gene expression were found between the two diet groups. Our study demonstrated that tamoxifen diet leads to decreased muscle atrophy and inflammatory changes following chronic rotator cuff tear, but has no apparent effect on adipogenesis.

Original languageEnglish (US)
Pages (from-to)1846-1853
Number of pages8
JournalJournal of Orthopaedic Research
Volume33
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Rotator Cuff
Tamoxifen
Atrophy
Animal Models
Muscles
Diet
Histology
Myostatin
Myogenin
Adipogenesis
Polymerase Chain Reaction
Peroxisome Proliferator-Activated Receptors
Muscular Atrophy
Adipocytes
Estrogen Receptors
Up-Regulation
Down-Regulation
Inflammation
Gene Expression
Rotator Cuff Injuries

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

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abstract = "Fatty degeneration of the rotator cuff muscles is an irreversible change resulting from chronic rotator cuff tear and is associated with poor clinical outcomes following rotator cuff repair. We evaluated the effect of Tamoxifen, a competitive estrogen receptor inhibitor, on fatty degeneration using a mouse model for chronic rotator cuff tear. Sixteen adult mice were divided into two diet groups (Tamoxifen vs. Regular) and subjected to surgical creation of a large rotator cuff tear and suprascapular nerve transection in their left shoulder with the right shoulder serving as a control. The rotator cuff muscles were harvested at 16 weeks and subjected to histology and RT-PCR for adipogenic and myogenic markers. Histology showed substantially decreased atrophy and endomysial inflammation in Tamoxifen group, but no significant differences in the amount of intramuscular adipocytes and lipid droplets compared to the Regular group. With RT-PCR, the operated shoulders showed significant upregulation of myogenin and PPAR-γ, and downregulation of myostatin compared to the nonsurgical shoulder. No significant differences of gene expression were found between the two diet groups. Our study demonstrated that tamoxifen diet leads to decreased muscle atrophy and inflammatory changes following chronic rotator cuff tear, but has no apparent effect on adipogenesis.",
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Effect of tamoxifen on fatty degeneration and atrophy of rotator cuff muscles in chronic rotator cuff tear : An animal model study. / Cho, Edward; Zhang, Yue; Pruznak, Anne; Kim, Hyun-Min Mike.

In: Journal of Orthopaedic Research, Vol. 33, No. 12, 01.12.2015, p. 1846-1853.

Research output: Contribution to journalArticle

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