Effect of temperature, carbon source, yeast extract, and pH on pullulan production by aureobasidium pullulans

Kuan Chen Cheng, Ali Demirci, Jeffrey Catchmark

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Pullulan, an exopolysaccharide produced by a yeast-like fungus Aureobasidium pullulans, has been used for various applications such as a blood plasma substitute, a food additive, an adhesive additive, and a flocculant. The goal of this study was to enhance the pullulan production by optimizing culture medium and pH profile on a batch fermentation with A. pullulans. Various carbon sources (sucrose, glucose, fructose, and lactose) and their concentrations, yeast extract concentration (3, 5, 7.5, 10 g/l), fermentation temperature (25, 28, 30, 32°C), and various pH profiles were examined. The optimal growth condition for pullulan production by A. pullulans has been found as 75 g/l of sucrose as carbon source, 3 g/l of yeast extract, and cultivation temperature at 30°C. Under these conditions with constant pH at 5, the final pullulan concentration was 19.3 g/l with 0.20 g/l/h maximum production rate. A bistage pH profile, which included an initial pH at 2.0 for the first 72 hours of fermentation and then switched to pH 5.0 (constant) until the end of fermentation, was used. The results demonstrated that pullulan production could be enhanced to 23.1 g/l after 7-d cultivation with 0.63 g/l/h maximum production rate.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009
Pages2394-2407
Number of pages14
StatePublished - Dec 1 2009
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009 - Reno, NV, United States
Duration: Jun 21 2009Jun 24 2009

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009
Volume4

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009
CountryUnited States
CityReno, NV
Period6/21/096/24/09

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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