TY - JOUR
T1 - Effect of the free radical-generating herbicide paraquat on the expression of the superoxide dismutase (Sod) genes in maize
AU - Matters, Gail L.
AU - Scandalios, John G.
N1 - Funding Information:
This research was supported in part by research grants GM22733 and GM33817 from the National Institutes of Health, U.S. Public Health Service, and the U.S. Environmental Protection Agency Grant No. R812404. This is paper No. 10177 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC.
PY - 1986/6/3
Y1 - 1986/6/3
N2 - 10-day-old maize leaves were treated with the oxygen free radical-generating herbicide paraquat for 12 h. Paraquat treatments (10-5 M) resulted in a 40% increase in superoxide dismutase (SOD) activity correlates with higher levels of specific isozymes. The chloroplast (SOD-1) and cytosolic (SOD-2 and SOD-4) forms were increased significantly; however, the mitochondrial form (SOD-3) was increased only slightly. Higher levels of SOD-4 and SOD-3 after paraquat exosure were the result of increased synthesis of these proteins, as determined by labeling in vivo with [35S]methionine. Isolation and in vitro translation of polysomes from 10-5 M paraquat-treated leaves indicated that paraquat increased the amount of polysomal mRNA which codes for SOD-4 and SOD-3. Superoxide dismutase induction does not appear to be a response that is specific to paraquat, since another superoxide-generating compound, juglone, caused a similar increase in total superoxide dismutase activity. Therefore, the effect of these compounds on the expression of the maize Sod genes is exerted via their ability to generate superoxide.
AB - 10-day-old maize leaves were treated with the oxygen free radical-generating herbicide paraquat for 12 h. Paraquat treatments (10-5 M) resulted in a 40% increase in superoxide dismutase (SOD) activity correlates with higher levels of specific isozymes. The chloroplast (SOD-1) and cytosolic (SOD-2 and SOD-4) forms were increased significantly; however, the mitochondrial form (SOD-3) was increased only slightly. Higher levels of SOD-4 and SOD-3 after paraquat exosure were the result of increased synthesis of these proteins, as determined by labeling in vivo with [35S]methionine. Isolation and in vitro translation of polysomes from 10-5 M paraquat-treated leaves indicated that paraquat increased the amount of polysomal mRNA which codes for SOD-4 and SOD-3. Superoxide dismutase induction does not appear to be a response that is specific to paraquat, since another superoxide-generating compound, juglone, caused a similar increase in total superoxide dismutase activity. Therefore, the effect of these compounds on the expression of the maize Sod genes is exerted via their ability to generate superoxide.
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U2 - 10.1016/0304-4165(86)90051-6
DO - 10.1016/0304-4165(86)90051-6
M3 - Article
C2 - 3707996
AN - SCOPUS:0022504269
VL - 882
SP - 29
EP - 38
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0006-3002
IS - 1
ER -