Effect of thyroid hormone on the abundance of na, K-adenosine triphosphatase α-subunit messenger ribonucleic acid

Sukanya Chaudhury, Faramarz Ismail-Beigi, Gregory G. Gick, Robert Levenson, Isidore S. Edelman

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The effects of thyroid hormone on Na, K-ATPase α-subunit mRNA (mRNAα) content and Na, K-ATPase activity were measured in renal cortex, heart, and cerebrum of hypothyroid rats 24 and 72 h after injection of diluent or T3. Use of a cDNA probe complementary to rat brain mRNAα in Northern blot analysis revealed a single 26-27 S band in RNA isolated from these three tissues regardless of thyroid status. Tissue mRNAα content was estimated by dot blot analysis of whole cell extracts and isolated total RNA. Injection of T3 augmented mRNAα content by 2.1- to 2.5-fold in kidney cortex and myocardium at 24 h. After three daily injections of T3, the increases in mRNAα were evident despite a global increase in RNA content associated with hypertrophy of these target tissues. Furthermore, the increases in abundance of mRNAα after 72 h of T3 treatment correlated with enhancement of Na, K-ATPase activity. In contrast, both mRNAα and enzyme activity were invariant in the cerebrum. These data suggest that T3-induced augmentation of Na, K-ATPase activity is mediated, at least in part, by increased mRNAα content in target tissues.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalMolecular Endocrinology
Volume1
Issue number1
DOIs
StatePublished - Jan 1987

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Effect of thyroid hormone on the abundance of na, K-adenosine triphosphatase α-subunit messenger ribonucleic acid'. Together they form a unique fingerprint.

Cite this