Effect of Tunicamycin on the Biosynthesis of Human Fibroblast Collagenase

Chu Chang Chua, Roger Ladda

Research output: Contribution to journalArticle

Abstract

It is generally accepted that collagenase from human fibroblast cultures consists of two proenzymes (Mr 60,000 and 55,000) and two active forms (Mr 50,000 and 43,000). We demonstrated previously that epidermal growth factor (EGF) as well as a number of other growth factors induced the secretion of procollagenase (Mr 60,000, Mr 55,000) into the medium of human fibroblast cultures (Chua et al., 1985). In the presence of tunicamycin and EGF, the secretion of the larger form of procollagenase was suppressed preferentially with concomitant appearance of a new band, Mr 40,000. This Mr 40,000 band could be specifically immunoprecipitated by antibody raised against human collagenase. By two-dimensional peptide mapping, the Mr 40,000 material appeared to have similar composition as the Mr 60,000 band. In a time course study, the Mr 55,000 procollagenase band was the earliest protein to appear in the medium after 1 hour labeling with [35S] -methionine. The Mr 60,000, 50,000 and 43,000 bands appeared after a 2 hour labeling period. Our results indicate that human collagenases are glycosylated proteins and are synthesized via the dolichol phosphate pathway.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalTopics in Catalysis
Volume7
Issue number4
DOIs
StatePublished - Jan 1 1987

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Matrix Metalloproteinase 8
Tunicamycin
Biosynthesis
Collagenases
Fibroblasts
Cell culture
Labeling
Proteins
Epidermal Growth Factor
Time and motion study
Dolichol Phosphates
Antibodies
Peptides
Enzyme Precursors
Phosphates
Methionine
Peptide Mapping
Intercellular Signaling Peptides and Proteins
Chemical analysis
procollagenase

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Rheumatology

Cite this

Chua, Chu Chang ; Ladda, Roger. / Effect of Tunicamycin on the Biosynthesis of Human Fibroblast Collagenase. In: Topics in Catalysis. 1987 ; Vol. 7, No. 4. pp. 285-293.
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Effect of Tunicamycin on the Biosynthesis of Human Fibroblast Collagenase. / Chua, Chu Chang; Ladda, Roger.

In: Topics in Catalysis, Vol. 7, No. 4, 01.01.1987, p. 285-293.

Research output: Contribution to journalArticle

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