Effect of ultraviolet c radiation on biological samples

Branka Gršković, Dario Zrnec, Maja Popović, Maja Jelena Petek, Dragan Primorac, Gordan Mršić

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim: To examine the influence of ultraviolet C (UVC) radiation on blood, saliva, semen, and naked DNA samples for preventing DNA cross-contamination on working surfaces in laboratories. Methods: Blood, saliva, semen, and DNA isolated from buccal swab samples were obtained from a single male donor and applied to the laboratory working surfaces. UVC radiation was applied to these diluted and undiluted samples with or without previous decontamination of the working surfaces with 10% sodium hypochlorite and 20% ethanol. Genomic DNA was extracted using Chelex. After quantification, DNA was amplified using the AmpFlSTR® NGMTM PCR Amplification Kit. We tested and statistically analyzed DNA concentration, UVCdose, sample volume, radiation time, the number of correctly detected alleles on genetic loci, and the number of correctly detected alleles in four groups in which 16 loci were divided. Results: When working surfaces were not decontaminated and were treated only with UVC radiation in the laboratory, the genetic profile for naked DNA could not be obtained after 2 minutes of UVC radiation and for saliva after 54 hours. For blood and semen, a partial genetic profile was obtained even after 250 hours of UVC radiation in the laminar. When working surfaces were decontaminated with 10% sodium hypochlorite and 20% ethanol, genetic profile could not be obtained for naked DNA after 2 minutes, for saliva after 4 hours, for blood after 16 hours, and for semen after 8 hours of UVC radiation in the laboratory. Conclusion: It is recommended to carefully and thoroughly clean working surfaces with 10% sodium hypochlorite and 20% ethanol followed by minimal 16-hour UVC exposure (dose approximately 4380 mJ/cm2) for complete and successful decontamination.

Original languageEnglish (US)
Pages (from-to)263-271
Number of pages9
JournalCroatian Medical Journal
Volume54
Issue number3
DOIs
StatePublished - Aug 20 2013

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Radiation
Semen
Saliva
DNA
Sodium Hypochlorite
Ethanol
Decontamination
DNA Contamination
Alleles
Genetic Loci
Cheek
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Gršković, B., Zrnec, D., Popović, M., Petek, M. J., Primorac, D., & Mršić, G. (2013). Effect of ultraviolet c radiation on biological samples. Croatian Medical Journal, 54(3), 263-271. https://doi.org/10.3325/cmj.2013.54.263
Gršković, Branka ; Zrnec, Dario ; Popović, Maja ; Petek, Maja Jelena ; Primorac, Dragan ; Mršić, Gordan. / Effect of ultraviolet c radiation on biological samples. In: Croatian Medical Journal. 2013 ; Vol. 54, No. 3. pp. 263-271.
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Gršković, B, Zrnec, D, Popović, M, Petek, MJ, Primorac, D & Mršić, G 2013, 'Effect of ultraviolet c radiation on biological samples', Croatian Medical Journal, vol. 54, no. 3, pp. 263-271. https://doi.org/10.3325/cmj.2013.54.263

Effect of ultraviolet c radiation on biological samples. / Gršković, Branka; Zrnec, Dario; Popović, Maja; Petek, Maja Jelena; Primorac, Dragan; Mršić, Gordan.

In: Croatian Medical Journal, Vol. 54, No. 3, 20.08.2013, p. 263-271.

Research output: Contribution to journalArticle

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AU - Gršković, Branka

AU - Zrnec, Dario

AU - Popović, Maja

AU - Petek, Maja Jelena

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AU - Mršić, Gordan

PY - 2013/8/20

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N2 - Aim: To examine the influence of ultraviolet C (UVC) radiation on blood, saliva, semen, and naked DNA samples for preventing DNA cross-contamination on working surfaces in laboratories. Methods: Blood, saliva, semen, and DNA isolated from buccal swab samples were obtained from a single male donor and applied to the laboratory working surfaces. UVC radiation was applied to these diluted and undiluted samples with or without previous decontamination of the working surfaces with 10% sodium hypochlorite and 20% ethanol. Genomic DNA was extracted using Chelex. After quantification, DNA was amplified using the AmpFlSTR® NGMTM PCR Amplification Kit. We tested and statistically analyzed DNA concentration, UVCdose, sample volume, radiation time, the number of correctly detected alleles on genetic loci, and the number of correctly detected alleles in four groups in which 16 loci were divided. Results: When working surfaces were not decontaminated and were treated only with UVC radiation in the laboratory, the genetic profile for naked DNA could not be obtained after 2 minutes of UVC radiation and for saliva after 54 hours. For blood and semen, a partial genetic profile was obtained even after 250 hours of UVC radiation in the laminar. When working surfaces were decontaminated with 10% sodium hypochlorite and 20% ethanol, genetic profile could not be obtained for naked DNA after 2 minutes, for saliva after 4 hours, for blood after 16 hours, and for semen after 8 hours of UVC radiation in the laboratory. Conclusion: It is recommended to carefully and thoroughly clean working surfaces with 10% sodium hypochlorite and 20% ethanol followed by minimal 16-hour UVC exposure (dose approximately 4380 mJ/cm2) for complete and successful decontamination.

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Gršković B, Zrnec D, Popović M, Petek MJ, Primorac D, Mršić G. Effect of ultraviolet c radiation on biological samples. Croatian Medical Journal. 2013 Aug 20;54(3):263-271. https://doi.org/10.3325/cmj.2013.54.263