Effective production of FXIIa and thrombin by model biomaterials

K. M. Bussard, E. A. Vogler, C. A. Siedlecki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The initiation of contact activation of the coagulation cascade takes place when Factor XII interacts with a procoagulant surface. Subsequent events include FXII autoactivation to the enzyme FXIIa and formation of a contact activation complex. Additional conversions result in the generation of thrombin and formation of an insoluble fibrin clot. This study examines the ability of a biomaterial to generate FXIIa and thrombin by comparing the procoagulant efficiency of glass beads to soluble aliquots of enzymes. FXIIa induced activation of coagulation saturates at surprisingly dilute concentrations (∼0.5 μg/ml), and this effect is also seen in surface and thrombin induced contact activation. Preliminary analysis of data suggests that 50 mm2 glass surface area generates the equivalent of 5 ng of FXIIa, or only ∼0.0125% conversion of physiologic concentration, as well as producing the equivalent of ∼2.65 units/ml of thrombin. Addition of procoagulant (glass) surface was also found to modulate a local minimum observed in FXIIa titration curves. The results observed with FXIIa and biomaterials suggest that an investigation of the mechanisms behind FXIIa and thrombin production by biomaterials, as well as a clearer understanding of how signals propagate down the pathway, are necessary for assembling a detailed mechanism for surface activation.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 28th Annual Northeast Bioengineering Conference, NEBC 2002
EditorsDalia El-Sherif, Karen Moxon, Saravanan Kanakasabai
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages129-130
Number of pages2
ISBN (Electronic)0780374193
DOIs
StatePublished - Jan 1 2002
Event28th IEEE Annual Northeast Bioengineering Conference, NEBC 2002 - Philadelphia, United States
Duration: Apr 20 2002Apr 21 2002

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume2002-January
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other28th IEEE Annual Northeast Bioengineering Conference, NEBC 2002
CountryUnited States
CityPhiladelphia
Period4/20/024/21/02

All Science Journal Classification (ASJC) codes

  • Bioengineering

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  • Cite this

    Bussard, K. M., Vogler, E. A., & Siedlecki, C. A. (2002). Effective production of FXIIa and thrombin by model biomaterials. In D. El-Sherif, K. Moxon, & S. Kanakasabai (Eds.), Proceedings of the IEEE 28th Annual Northeast Bioengineering Conference, NEBC 2002 (pp. 129-130). [999499] (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC; Vol. 2002-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEBC.2002.999499