Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source

Mohan Ganesh, Arivazhagan Senthamarai, Shanmughapriya Santhanam, Kalimuthusamy Natarajaseenivasan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Utilization of bio-diesel by-products (glycerol) for microbial polymer production has created a novel biorefinery concept. In the present study, recombinant Escherichia coli JM109 was used for the production of P(3HB) from glycerol as carbon source. Batch fermentation in a 7.5L bioreactor with the statistically optimized culture condition (pre-treated glycerol: 27.5g/L and casein hydrolysate: 5.25g/L) scaled up the P3HB production to 65% (~8g/L). FTIR, 1H and 13C NMR analysis proved the polymer produced to be P(3HB). Gel permeation chromatography, Differential Scanning Calorimetry (DSC) and thermogravimetric analysis (TGA) demonstrated the produced P(3HB) to have high molecular weight (2.84×106) and lowered crystallinity (~30%) compared to commercial polymer. Integrating the production efficiency and the thermal characteristics of the polymer produced by recombinant E. coli, the viability and sustainability of biofuels and biopolymers for economic human need could be enhanced.

Original languageEnglish (US)
Pages (from-to)677-681
Number of pages5
JournalBioresource technology
Volume192
DOIs
StatePublished - Sep 1 2015

Fingerprint

crystallinity
Glycerol
Escherichia coli
Polymers
Carbon
polymer
carbon
Casein
Biopolymers
Biofuels
calorimetry
Gel permeation chromatography
Biodiesel
Bioreactors
biofuel
bioreactor
Fermentation
chromatography
fermentation
diesel

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

@article{bad95b45bce746a68a92bc3a1c319be8,
title = "Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source",
abstract = "Utilization of bio-diesel by-products (glycerol) for microbial polymer production has created a novel biorefinery concept. In the present study, recombinant Escherichia coli JM109 was used for the production of P(3HB) from glycerol as carbon source. Batch fermentation in a 7.5L bioreactor with the statistically optimized culture condition (pre-treated glycerol: 27.5g/L and casein hydrolysate: 5.25g/L) scaled up the P3HB production to 65{\%} (~8g/L). FTIR, 1H and 13C NMR analysis proved the polymer produced to be P(3HB). Gel permeation chromatography, Differential Scanning Calorimetry (DSC) and thermogravimetric analysis (TGA) demonstrated the produced P(3HB) to have high molecular weight (2.84×106) and lowered crystallinity (~30{\%}) compared to commercial polymer. Integrating the production efficiency and the thermal characteristics of the polymer produced by recombinant E. coli, the viability and sustainability of biofuels and biopolymers for economic human need could be enhanced.",
author = "Mohan Ganesh and Arivazhagan Senthamarai and Shanmughapriya Santhanam and Kalimuthusamy Natarajaseenivasan",
year = "2015",
month = "9",
day = "1",
doi = "10.1016/j.biortech.2015.06.042",
language = "English (US)",
volume = "192",
pages = "677--681",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",

}

Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source. / Ganesh, Mohan; Senthamarai, Arivazhagan; Santhanam, Shanmughapriya; Natarajaseenivasan, Kalimuthusamy.

In: Bioresource technology, Vol. 192, 01.09.2015, p. 677-681.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source

AU - Ganesh, Mohan

AU - Senthamarai, Arivazhagan

AU - Santhanam, Shanmughapriya

AU - Natarajaseenivasan, Kalimuthusamy

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Utilization of bio-diesel by-products (glycerol) for microbial polymer production has created a novel biorefinery concept. In the present study, recombinant Escherichia coli JM109 was used for the production of P(3HB) from glycerol as carbon source. Batch fermentation in a 7.5L bioreactor with the statistically optimized culture condition (pre-treated glycerol: 27.5g/L and casein hydrolysate: 5.25g/L) scaled up the P3HB production to 65% (~8g/L). FTIR, 1H and 13C NMR analysis proved the polymer produced to be P(3HB). Gel permeation chromatography, Differential Scanning Calorimetry (DSC) and thermogravimetric analysis (TGA) demonstrated the produced P(3HB) to have high molecular weight (2.84×106) and lowered crystallinity (~30%) compared to commercial polymer. Integrating the production efficiency and the thermal characteristics of the polymer produced by recombinant E. coli, the viability and sustainability of biofuels and biopolymers for economic human need could be enhanced.

AB - Utilization of bio-diesel by-products (glycerol) for microbial polymer production has created a novel biorefinery concept. In the present study, recombinant Escherichia coli JM109 was used for the production of P(3HB) from glycerol as carbon source. Batch fermentation in a 7.5L bioreactor with the statistically optimized culture condition (pre-treated glycerol: 27.5g/L and casein hydrolysate: 5.25g/L) scaled up the P3HB production to 65% (~8g/L). FTIR, 1H and 13C NMR analysis proved the polymer produced to be P(3HB). Gel permeation chromatography, Differential Scanning Calorimetry (DSC) and thermogravimetric analysis (TGA) demonstrated the produced P(3HB) to have high molecular weight (2.84×106) and lowered crystallinity (~30%) compared to commercial polymer. Integrating the production efficiency and the thermal characteristics of the polymer produced by recombinant E. coli, the viability and sustainability of biofuels and biopolymers for economic human need could be enhanced.

UR - http://www.scopus.com/inward/record.url?scp=84934783638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934783638&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2015.06.042

DO - 10.1016/j.biortech.2015.06.042

M3 - Article

VL - 192

SP - 677

EP - 681

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -