TY - JOUR
T1 - Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source
AU - Ganesh, Mohan
AU - Senthamarai, Arivazhagan
AU - Shanmughapriya, Santhanam
AU - Natarajaseenivasan, Kalimuthusamy
N1 - Funding Information:
We acknowledge the Department of Science and Technology (DST), Government of India for the Research and Development grant (DST/IS-STAC/CO2-SR-75/10). We extent our thanks to CEESAT, National Institute of Technology (NIT), Tiruchirappalli for the DSC, TGC and FTIR analysis, Central Electrochemical Research Institute (CECRI), Karaikudi for NMR and Charutar Vidya Mandal’s Sophisticated Instrumentation Centre for Applied Research and Testing (SICART) for GPC analysis. The authors also thank Dr. K. Srinivasan, Department of Chemistry, Bharathidasan University for his decisive advice on the structural and thermal characterization of the polymer.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Utilization of bio-diesel by-products (glycerol) for microbial polymer production has created a novel biorefinery concept. In the present study, recombinant Escherichia coli JM109 was used for the production of P(3HB) from glycerol as carbon source. Batch fermentation in a 7.5L bioreactor with the statistically optimized culture condition (pre-treated glycerol: 27.5g/L and casein hydrolysate: 5.25g/L) scaled up the P3HB production to 65% (~8g/L). FTIR, 1H and 13C NMR analysis proved the polymer produced to be P(3HB). Gel permeation chromatography, Differential Scanning Calorimetry (DSC) and thermogravimetric analysis (TGA) demonstrated the produced P(3HB) to have high molecular weight (2.84×106) and lowered crystallinity (~30%) compared to commercial polymer. Integrating the production efficiency and the thermal characteristics of the polymer produced by recombinant E. coli, the viability and sustainability of biofuels and biopolymers for economic human need could be enhanced.
AB - Utilization of bio-diesel by-products (glycerol) for microbial polymer production has created a novel biorefinery concept. In the present study, recombinant Escherichia coli JM109 was used for the production of P(3HB) from glycerol as carbon source. Batch fermentation in a 7.5L bioreactor with the statistically optimized culture condition (pre-treated glycerol: 27.5g/L and casein hydrolysate: 5.25g/L) scaled up the P3HB production to 65% (~8g/L). FTIR, 1H and 13C NMR analysis proved the polymer produced to be P(3HB). Gel permeation chromatography, Differential Scanning Calorimetry (DSC) and thermogravimetric analysis (TGA) demonstrated the produced P(3HB) to have high molecular weight (2.84×106) and lowered crystallinity (~30%) compared to commercial polymer. Integrating the production efficiency and the thermal characteristics of the polymer produced by recombinant E. coli, the viability and sustainability of biofuels and biopolymers for economic human need could be enhanced.
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U2 - 10.1016/j.biortech.2015.06.042
DO - 10.1016/j.biortech.2015.06.042
M3 - Article
C2 - 26094193
AN - SCOPUS:84934783638
SN - 0960-8524
VL - 192
SP - 677
EP - 681
JO - Agricultural Wastes
JF - Agricultural Wastes
ER -
