Effects of a Mediterranean Diet Intervention on Anti- and Pro-Inflammatory Eicosanoids, Epithelial Proliferation, and Nuclear Morphology in Biopsies of Normal Colon Tissue

Zora Djuric, D. Kim Turgeon, Jianwei Ren, Andrew Neilson, Missy Plegue, Ian G. Waters, Alexander Chan, Leah M. Askew, Mack Ruffin, Ananda Sen, Dean E. Brenner

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This randomized trial evaluated the effects of intervention with either a Healthy Eating or a Mediterranean diet on colon biomarkers in 120 healthy individuals at increased colon cancer risk. The hypothesis was that eicosanoids and markers of proliferation would be favorably affected by the Mediterranean diet. Colon epithelial biopsy tissues and blood samples were obtained at baseline and after 6 mo of intervention. Colonic eicosanoid concentrations were evaluated by HPLC-MS-MS, and measures of epithelial proliferation and nuclear morphology were evaluated by image analysis of biopsy sections. There was little change in proinflammatory eicosanoids and in plasma cytokine concentrations with either dietary intervention. There was, however, a 50% increase in colonic prostaglandin E3 (PGE3), which is formed from eicosapentanoic acid, in the Mediterranean arm. Unlike PGE2, PGE3, was not significantly affected by regular use of non-steroidal anti-inflammatory drugs at baseline, and normal weight subjects had significantly higher colon PGE3 than overweight or obese subjects. Increased proliferation in the colon at baseline, by Ki67 labeling, was associated with morphological features that defined smaller nuclei in the epithelial cells, lower colon leukotriene concentrations and higher plasma cytokine concentrations. Dietary intervention had little effect on measures of epithelial proliferation or of nuclear morphology. The increase in PGE3 with a Mediterranean diet indicates that in normal colon, diet might affect protective pathways to a greater extent than proinflammatory and proliferative pathways. Hence, biomarkers from cancer models might not be relevant in a true prevention setting.

Original languageEnglish (US)
Pages (from-to)721-729
Number of pages9
JournalNutrition and cancer
Volume67
Issue number5
DOIs
StatePublished - Jul 4 2015

Fingerprint

Mediterranean Diet
Eicosanoids
Colon
Anti-Inflammatory Agents
Biopsy
Cytokines
Eicosapentaenoic Acid
Leukotrienes
Tumor Biomarkers
Dinoprostone
Colonic Neoplasms
Epithelium
Biomarkers
Epithelial Cells
High Pressure Liquid Chromatography
Diet
Weights and Measures
prostaglandin E3
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Djuric, Zora ; Turgeon, D. Kim ; Ren, Jianwei ; Neilson, Andrew ; Plegue, Missy ; Waters, Ian G. ; Chan, Alexander ; Askew, Leah M. ; Ruffin, Mack ; Sen, Ananda ; Brenner, Dean E. / Effects of a Mediterranean Diet Intervention on Anti- and Pro-Inflammatory Eicosanoids, Epithelial Proliferation, and Nuclear Morphology in Biopsies of Normal Colon Tissue. In: Nutrition and cancer. 2015 ; Vol. 67, No. 5. pp. 721-729.
@article{fe4fed1017c0448b9a2c73824aefc48e,
title = "Effects of a Mediterranean Diet Intervention on Anti- and Pro-Inflammatory Eicosanoids, Epithelial Proliferation, and Nuclear Morphology in Biopsies of Normal Colon Tissue",
abstract = "This randomized trial evaluated the effects of intervention with either a Healthy Eating or a Mediterranean diet on colon biomarkers in 120 healthy individuals at increased colon cancer risk. The hypothesis was that eicosanoids and markers of proliferation would be favorably affected by the Mediterranean diet. Colon epithelial biopsy tissues and blood samples were obtained at baseline and after 6 mo of intervention. Colonic eicosanoid concentrations were evaluated by HPLC-MS-MS, and measures of epithelial proliferation and nuclear morphology were evaluated by image analysis of biopsy sections. There was little change in proinflammatory eicosanoids and in plasma cytokine concentrations with either dietary intervention. There was, however, a 50{\%} increase in colonic prostaglandin E3 (PGE3), which is formed from eicosapentanoic acid, in the Mediterranean arm. Unlike PGE2, PGE3, was not significantly affected by regular use of non-steroidal anti-inflammatory drugs at baseline, and normal weight subjects had significantly higher colon PGE3 than overweight or obese subjects. Increased proliferation in the colon at baseline, by Ki67 labeling, was associated with morphological features that defined smaller nuclei in the epithelial cells, lower colon leukotriene concentrations and higher plasma cytokine concentrations. Dietary intervention had little effect on measures of epithelial proliferation or of nuclear morphology. The increase in PGE3 with a Mediterranean diet indicates that in normal colon, diet might affect protective pathways to a greater extent than proinflammatory and proliferative pathways. Hence, biomarkers from cancer models might not be relevant in a true prevention setting.",
author = "Zora Djuric and Turgeon, {D. Kim} and Jianwei Ren and Andrew Neilson and Missy Plegue and Waters, {Ian G.} and Alexander Chan and Askew, {Leah M.} and Mack Ruffin and Ananda Sen and Brenner, {Dean E.}",
year = "2015",
month = "7",
day = "4",
doi = "10.1080/01635581.2015.1029637",
language = "English (US)",
volume = "67",
pages = "721--729",
journal = "Nutrition and Cancer",
issn = "0163-5581",
publisher = "Routledge",
number = "5",

}

Effects of a Mediterranean Diet Intervention on Anti- and Pro-Inflammatory Eicosanoids, Epithelial Proliferation, and Nuclear Morphology in Biopsies of Normal Colon Tissue. / Djuric, Zora; Turgeon, D. Kim; Ren, Jianwei; Neilson, Andrew; Plegue, Missy; Waters, Ian G.; Chan, Alexander; Askew, Leah M.; Ruffin, Mack; Sen, Ananda; Brenner, Dean E.

In: Nutrition and cancer, Vol. 67, No. 5, 04.07.2015, p. 721-729.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of a Mediterranean Diet Intervention on Anti- and Pro-Inflammatory Eicosanoids, Epithelial Proliferation, and Nuclear Morphology in Biopsies of Normal Colon Tissue

AU - Djuric, Zora

AU - Turgeon, D. Kim

AU - Ren, Jianwei

AU - Neilson, Andrew

AU - Plegue, Missy

AU - Waters, Ian G.

AU - Chan, Alexander

AU - Askew, Leah M.

AU - Ruffin, Mack

AU - Sen, Ananda

AU - Brenner, Dean E.

PY - 2015/7/4

Y1 - 2015/7/4

N2 - This randomized trial evaluated the effects of intervention with either a Healthy Eating or a Mediterranean diet on colon biomarkers in 120 healthy individuals at increased colon cancer risk. The hypothesis was that eicosanoids and markers of proliferation would be favorably affected by the Mediterranean diet. Colon epithelial biopsy tissues and blood samples were obtained at baseline and after 6 mo of intervention. Colonic eicosanoid concentrations were evaluated by HPLC-MS-MS, and measures of epithelial proliferation and nuclear morphology were evaluated by image analysis of biopsy sections. There was little change in proinflammatory eicosanoids and in plasma cytokine concentrations with either dietary intervention. There was, however, a 50% increase in colonic prostaglandin E3 (PGE3), which is formed from eicosapentanoic acid, in the Mediterranean arm. Unlike PGE2, PGE3, was not significantly affected by regular use of non-steroidal anti-inflammatory drugs at baseline, and normal weight subjects had significantly higher colon PGE3 than overweight or obese subjects. Increased proliferation in the colon at baseline, by Ki67 labeling, was associated with morphological features that defined smaller nuclei in the epithelial cells, lower colon leukotriene concentrations and higher plasma cytokine concentrations. Dietary intervention had little effect on measures of epithelial proliferation or of nuclear morphology. The increase in PGE3 with a Mediterranean diet indicates that in normal colon, diet might affect protective pathways to a greater extent than proinflammatory and proliferative pathways. Hence, biomarkers from cancer models might not be relevant in a true prevention setting.

AB - This randomized trial evaluated the effects of intervention with either a Healthy Eating or a Mediterranean diet on colon biomarkers in 120 healthy individuals at increased colon cancer risk. The hypothesis was that eicosanoids and markers of proliferation would be favorably affected by the Mediterranean diet. Colon epithelial biopsy tissues and blood samples were obtained at baseline and after 6 mo of intervention. Colonic eicosanoid concentrations were evaluated by HPLC-MS-MS, and measures of epithelial proliferation and nuclear morphology were evaluated by image analysis of biopsy sections. There was little change in proinflammatory eicosanoids and in plasma cytokine concentrations with either dietary intervention. There was, however, a 50% increase in colonic prostaglandin E3 (PGE3), which is formed from eicosapentanoic acid, in the Mediterranean arm. Unlike PGE2, PGE3, was not significantly affected by regular use of non-steroidal anti-inflammatory drugs at baseline, and normal weight subjects had significantly higher colon PGE3 than overweight or obese subjects. Increased proliferation in the colon at baseline, by Ki67 labeling, was associated with morphological features that defined smaller nuclei in the epithelial cells, lower colon leukotriene concentrations and higher plasma cytokine concentrations. Dietary intervention had little effect on measures of epithelial proliferation or of nuclear morphology. The increase in PGE3 with a Mediterranean diet indicates that in normal colon, diet might affect protective pathways to a greater extent than proinflammatory and proliferative pathways. Hence, biomarkers from cancer models might not be relevant in a true prevention setting.

UR - http://www.scopus.com/inward/record.url?scp=84934443359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934443359&partnerID=8YFLogxK

U2 - 10.1080/01635581.2015.1029637

DO - 10.1080/01635581.2015.1029637

M3 - Article

C2 - 25869112

AN - SCOPUS:84934443359

VL - 67

SP - 721

EP - 729

JO - Nutrition and Cancer

JF - Nutrition and Cancer

SN - 0163-5581

IS - 5

ER -