Effects of a pressure release on virus retention with the Ultipor DV20 membrane

Melissa A. Woods, Andrew L. Zydney

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Recent data have shown that a temporary release in the transmembrane pressure can cause a significant increase in virus transmission through a number of commercial virus filters. The objective of this work was to study the effect of a pressure release on retention of the bacteriophage ΦX174 by the Ultipor DV20 membrane, with phage capture within the membrane visualized by confocal microscopy. Phage challenge tests showed a significant transient increase in phage transmission (typically by an order of magnitude) immediately after a pressure release. This transient increase was not due to any damage to the filter or to the presence of a back-flow (from permeate to feed). Confocal images demonstrated that the pressure release caused the migration of previously captured bacteriophage further into the depth of the filter. Data were analyzed using a modified internal polarization model, with the results providing important insights into the factors controlling virus retention during virus filtration.

Original languageEnglish (US)
Pages (from-to)545-551
Number of pages7
JournalBiotechnology and bioengineering
Volume111
Issue number3
DOIs
StatePublished - Mar 1 2014

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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