Effects of black raspberry extract and protocatechuic acid on carcinogen-DNA adducts and mutagenesis, and oxidative stress in rat and human oral cells

Joseph B. Guttenplan, Kun Ming Chen, Yuan Wan Sun, Wieslawa Kosinska, Ying Zhou, Seungjin Agatha Kim, Youngjae Sung, Krishne Gowda, Shantu Amin, Gary D. Stoner, Karam El-Bayoumy

Research output: Contribution to journalArticle

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Abstract

Effects of black raspberry (BRB) extract and protocatechuic acid (PCA) on DNA adduct formation and mutagenesis induced by metabolites of dibenzo[a,l]pyrene (DBP) were investigated in rat oral fibroblasts. The DBP metabolites, -anti-11,12-dihydroxy-11,12,-dihydrodibenzo[a,l]pyrene (DBP-diol) and 11,12-dihydroxy-13,14-epoxy-11,12,13,14-tetrahydrodibenzo[a,l]pyrene (DBPDE) induced dose-dependent DNA adducts and mutations. DBPDE was considerably more potent, whereas the parent compound had no significant effect. Treatment with BRB extract (BRBE) and PCA resulted in reduced DBP-derived DNA adduct levels and reduced mutagenesis induced by DBP-diol, but only BRBE was similarly effective against (DBPDE). BRBE did not directly inactivate DBPDE, but rather induced a cellular response enhanced DNA repair. When BRBE was added to cells 1 day after the DBP-diol, the BRBE greatly enhanced removal of DBP-derived DNA adducts. As oxidative stress can contribute to several stages of carcinogenesis, BRBE and PCA were investigated for their abilities to reduce oxidative stress in a human leukoplakia cell line by monitoring the redox indicator, 20,70-dichlorodihydrofluorescein diacetate (H2DCF) in cellular and acellular systems. BRBE effectively inhibited the oxidation, but PCA was only minimally effective against H2DCF. These results taken together provide evidence that BRBE and PCA can inhibit initiation of carcinogenesis by polycyclic aromatic hydrocarbons; and in addition, BRBE reduces oxidative stress.

Original languageEnglish (US)
Pages (from-to)704-712
Number of pages9
JournalCancer Prevention Research
Volume9
Issue number8
DOIs
StatePublished - Aug 2016

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DNA Adducts
Mutagenesis
Carcinogens
Oxidative Stress
Carcinogenesis
Leukoplakia
Polycyclic Aromatic Hydrocarbons
DNA Repair
Oxidation-Reduction
Fibroblasts
Rubus
protocatechuic acid
Cell Line
Mutation

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

@article{00070805e8ae4dec94e86216a85d641c,
title = "Effects of black raspberry extract and protocatechuic acid on carcinogen-DNA adducts and mutagenesis, and oxidative stress in rat and human oral cells",
abstract = "Effects of black raspberry (BRB) extract and protocatechuic acid (PCA) on DNA adduct formation and mutagenesis induced by metabolites of dibenzo[a,l]pyrene (DBP) were investigated in rat oral fibroblasts. The DBP metabolites, -anti-11,12-dihydroxy-11,12,-dihydrodibenzo[a,l]pyrene (DBP-diol) and 11,12-dihydroxy-13,14-epoxy-11,12,13,14-tetrahydrodibenzo[a,l]pyrene (DBPDE) induced dose-dependent DNA adducts and mutations. DBPDE was considerably more potent, whereas the parent compound had no significant effect. Treatment with BRB extract (BRBE) and PCA resulted in reduced DBP-derived DNA adduct levels and reduced mutagenesis induced by DBP-diol, but only BRBE was similarly effective against (DBPDE). BRBE did not directly inactivate DBPDE, but rather induced a cellular response enhanced DNA repair. When BRBE was added to cells 1 day after the DBP-diol, the BRBE greatly enhanced removal of DBP-derived DNA adducts. As oxidative stress can contribute to several stages of carcinogenesis, BRBE and PCA were investigated for their abilities to reduce oxidative stress in a human leukoplakia cell line by monitoring the redox indicator, 20,70-dichlorodihydrofluorescein diacetate (H2DCF) in cellular and acellular systems. BRBE effectively inhibited the oxidation, but PCA was only minimally effective against H2DCF. These results taken together provide evidence that BRBE and PCA can inhibit initiation of carcinogenesis by polycyclic aromatic hydrocarbons; and in addition, BRBE reduces oxidative stress.",
author = "Guttenplan, {Joseph B.} and Chen, {Kun Ming} and Sun, {Yuan Wan} and Wieslawa Kosinska and Ying Zhou and Kim, {Seungjin Agatha} and Youngjae Sung and Krishne Gowda and Shantu Amin and Stoner, {Gary D.} and Karam El-Bayoumy",
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Effects of black raspberry extract and protocatechuic acid on carcinogen-DNA adducts and mutagenesis, and oxidative stress in rat and human oral cells. / Guttenplan, Joseph B.; Chen, Kun Ming; Sun, Yuan Wan; Kosinska, Wieslawa; Zhou, Ying; Kim, Seungjin Agatha; Sung, Youngjae; Gowda, Krishne; Amin, Shantu; Stoner, Gary D.; El-Bayoumy, Karam.

In: Cancer Prevention Research, Vol. 9, No. 8, 08.2016, p. 704-712.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of black raspberry extract and protocatechuic acid on carcinogen-DNA adducts and mutagenesis, and oxidative stress in rat and human oral cells

AU - Guttenplan, Joseph B.

AU - Chen, Kun Ming

AU - Sun, Yuan Wan

AU - Kosinska, Wieslawa

AU - Zhou, Ying

AU - Kim, Seungjin Agatha

AU - Sung, Youngjae

AU - Gowda, Krishne

AU - Amin, Shantu

AU - Stoner, Gary D.

AU - El-Bayoumy, Karam

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