Effects of contraction and lactic acid on the discharge of group III muscle afferents in cats

L. I. Sinoway, J. M. Hill, J. G. Pickar, M. P. Kaufman

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Abstract

1. In barbiturate-anesthetized cats we examined the interaction of lactic acid and static contraction on the discharge of group III muscle afferents. Only afferents whose receptive fields were located in the triceps surae muscles were studied. 2. Twelve of 20 afferents were stimulated by a 60-s static contraction. The majority of firing occurred within the first few seconds of contraction. Thirteen of 20 afferents were stimulated by femoral arterial injections of 24 mM lactic acid (1-4 ml) with the muscle at rest. Repeated injections of lactic acid with the muscle at rest led to tachyphylaxis. Lactic acid was then injected (24 mM; 4 ml) during the last 15 s of static contraction. In eight of nine afferents that were tachyphylactic to lactic acid with the muscle at rest, we noted a restored sensitivity to lactic acid during contraction. 3. In separate experiments we examined the effects of dichloroacetate (DCA) on the responses of group III muscle afferents to static contraction. DCA reduces the production of lactic acid by increasing levels of the active form of the enzyme pyruvate dehydrogenase. 4. DCA lowered arterial and venous lactate concentrations at rest and during contraction. DCA significantly decreased (31%; P < 0.05) the responses of the afferents to contraction. This effect was most prominent within the first 10 s of contraction and was not due to a reduced level of mechanical stimulation after DCA, because peak tension levels were the same during the two bouts of contraction. 5. In conclusion, these experiments further characterize the important role lactic acid plays in stimulating group III muscle afferents during contraction. Increased delivery of lactic acid in the absence of contraction enhances their discharge, and reduced lactic acid levels during contraction decreases their discharge. In addition, repeated exposure of the afferent to lactic acid leads to a reduction in discharge frequency, which can be reversed by muscle contraction.

Original languageEnglish (US)
Pages (from-to)1053-1059
Number of pages7
JournalJournal of neurophysiology
Volume69
Issue number4
DOIs
Publication statusPublished - Jan 1 1993

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

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