Effects of Delignification on Crystalline Cellulose in Lignocellulose Biomass Characterized by Vibrational Sum Frequency Generation Spectroscopy and X-ray Diffraction

Kabindra Kafle, Christopher M. Lee, Heenae Shin, Justin Zoppe, David K. Johnson, Seong Kim, Sunkyu Park

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Delignification, a common practice in the pulping industry, has been proposed and explored as a means to selectively remove lignin from lignocellulosic biomass and, thus, increase enzyme accessibility for cellulose hydrolysis. However, without knowing structural changes of cellulose in biomass, it is difficult to fully understand the effects of the delignification process on cellulose hydrolysis. In this study, the amount and aggregation of crystalline cellulose in hardwood biomass delignified using oxygen and sodium chlorite as reactive agents were examined with vibrational sum frequency generation (SFG) spectroscopy and X-ray diffraction (XRD). The results indicated that the amount of crystalline cellulose and the XRD crystallite size increased with both oxygen and chlorite delignification processes. In addition, the “α-cellulose equivalent” fraction estimated by SFG spectroscopy increased greater than glucan amount with the delignification process. Changes in crystal size might be due to the aggregation of cellulose crystals, along with the increase in crystalline cellulose amount.

Original languageEnglish (US)
Pages (from-to)1750-1758
Number of pages9
JournalBioenergy Research
Volume8
Issue number4
DOIs
StatePublished - Dec 1 2015

Fingerprint

delignification
lignocellulose
Delignification
Vibrational spectra
X-ray diffraction
spectroscopy
Cellulose
cellulose
Biomass
Spectroscopy
Crystalline materials
X ray diffraction
biomass
chlorites
crystals
Hydrolysis
Agglomeration
hydrolysis
oxygen
pulping

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Energy (miscellaneous)

Cite this

Kafle, Kabindra ; Lee, Christopher M. ; Shin, Heenae ; Zoppe, Justin ; Johnson, David K. ; Kim, Seong ; Park, Sunkyu. / Effects of Delignification on Crystalline Cellulose in Lignocellulose Biomass Characterized by Vibrational Sum Frequency Generation Spectroscopy and X-ray Diffraction. In: Bioenergy Research. 2015 ; Vol. 8, No. 4. pp. 1750-1758.
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abstract = "Delignification, a common practice in the pulping industry, has been proposed and explored as a means to selectively remove lignin from lignocellulosic biomass and, thus, increase enzyme accessibility for cellulose hydrolysis. However, without knowing structural changes of cellulose in biomass, it is difficult to fully understand the effects of the delignification process on cellulose hydrolysis. In this study, the amount and aggregation of crystalline cellulose in hardwood biomass delignified using oxygen and sodium chlorite as reactive agents were examined with vibrational sum frequency generation (SFG) spectroscopy and X-ray diffraction (XRD). The results indicated that the amount of crystalline cellulose and the XRD crystallite size increased with both oxygen and chlorite delignification processes. In addition, the “α-cellulose equivalent” fraction estimated by SFG spectroscopy increased greater than glucan amount with the delignification process. Changes in crystal size might be due to the aggregation of cellulose crystals, along with the increase in crystalline cellulose amount.",
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Effects of Delignification on Crystalline Cellulose in Lignocellulose Biomass Characterized by Vibrational Sum Frequency Generation Spectroscopy and X-ray Diffraction. / Kafle, Kabindra; Lee, Christopher M.; Shin, Heenae; Zoppe, Justin; Johnson, David K.; Kim, Seong; Park, Sunkyu.

In: Bioenergy Research, Vol. 8, No. 4, 01.12.2015, p. 1750-1758.

Research output: Contribution to journalArticle

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AU - Lee, Christopher M.

AU - Shin, Heenae

AU - Zoppe, Justin

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AU - Park, Sunkyu

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