Effects of dietary selenium and vitamin e on red blood cell peroxidation, glutathione peroxidase activity, and macrophage superoxide anion production in channel catfish

D. J. Wise, J. R. Tomasso, D. M. Gatlin, S. C. Bai, Vicki Suzette Blazer

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Fingerling channel catfish Iclalurus punctatus were fed purified diets either unsupplemented and deficient in both selenium and vitamin E, deficient in either selenium or vitamin E, adequate in both selenium (0.2 mg/kg) and vitamin E (60 mg/kg), or excessive in both nutrients (four times the recommended levels). After 115-120 d of feeding the experimental diets, the red blood cell membranes offish fed diets deficient in vitamin E were more susceptible to peroxidation than were those of fish fed diets that met or exceeded vitamin E recommendations. Hepatic, selenium-dependent glutathione peroxidase activity was suppressed in fish fed diets deficient in selenium when compared with this enzyme activity in fish fed diets containing recommended or higher levels of selenium. Intracellular superoxide anion production of macrophages was higher in fish fed the diet fortified with four times the recommended levels of both nutrients than in fish fed the other diets. None of the results indicated that selenium and vitamin E were complementing each other or that one nutrient was compensating for a deficiency of the other. The results of this study support the presently recommended levels of selenium and vitamin E for channel catfish diets and indicate that higher-than-recommended levels of one or both nutrients may enhance macrophage function.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalJournal of Aquatic Animal Health
Volume5
Issue number3
DOIs
StatePublished - Jan 1 1993

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peroxidation
Ictalurus punctatus
vitamin
selenium
glutathione peroxidase
superoxide anion
anion
vitamins
macrophages
erythrocytes
vitamin E
blood
diet
fish feeds
fish
nutrients
nutrient
effect
fingerlings
experimental diets

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

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title = "Effects of dietary selenium and vitamin e on red blood cell peroxidation, glutathione peroxidase activity, and macrophage superoxide anion production in channel catfish",
abstract = "Fingerling channel catfish Iclalurus punctatus were fed purified diets either unsupplemented and deficient in both selenium and vitamin E, deficient in either selenium or vitamin E, adequate in both selenium (0.2 mg/kg) and vitamin E (60 mg/kg), or excessive in both nutrients (four times the recommended levels). After 115-120 d of feeding the experimental diets, the red blood cell membranes offish fed diets deficient in vitamin E were more susceptible to peroxidation than were those of fish fed diets that met or exceeded vitamin E recommendations. Hepatic, selenium-dependent glutathione peroxidase activity was suppressed in fish fed diets deficient in selenium when compared with this enzyme activity in fish fed diets containing recommended or higher levels of selenium. Intracellular superoxide anion production of macrophages was higher in fish fed the diet fortified with four times the recommended levels of both nutrients than in fish fed the other diets. None of the results indicated that selenium and vitamin E were complementing each other or that one nutrient was compensating for a deficiency of the other. The results of this study support the presently recommended levels of selenium and vitamin E for channel catfish diets and indicate that higher-than-recommended levels of one or both nutrients may enhance macrophage function.",
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Effects of dietary selenium and vitamin e on red blood cell peroxidation, glutathione peroxidase activity, and macrophage superoxide anion production in channel catfish. / Wise, D. J.; Tomasso, J. R.; Gatlin, D. M.; Bai, S. C.; Blazer, Vicki Suzette.

In: Journal of Aquatic Animal Health, Vol. 5, No. 3, 01.01.1993, p. 177-182.

Research output: Contribution to journalArticle

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