Effects of essential oils on in vitro ruminal fermentation and ammonia release

Alexander Nikolov Hristov, J. K. Ropp, S. Zaman, A. Melgar

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ruminal inoculum enriched with particle-associated microorganisms was collected from two lactating dairy cows fed an alfalfa hay/cereal silage/concentrate diet 1 h before feeding and used to evaluate effects of essential oils (EO) on ruminal fermentation in short-term in vitro incubations. Ruminal ammonia N was labeled with 15N and native and hydrolyzed casein were provided as sources of amino acids. Forty EO were tested at 10 and 100 mg/l final medium concentration. Monensin-Na, and sodium laurate were also incubated at 5 and 2000 mg/l, respectively. Compared with blanks (i.e., no addition of EO), sodium laurate increased medium pH and a number of EO reduced medium pH. Both sodium laurate and monensin reduced ammonia concentrations compared to the blank. Only one of the tested EO (i.e., Caraway) slightly reduced ammonia concentration, by 8%, compared with the blank. Monensin and sodium laurate resulted in higher (i.e., 9-34%, monensin, and 29-47%, sodium laurate) 15N enrichment of ammonia N, an indication of reduced deamination of amino acids in these treatments versus the blank. Several EO (i.e., FrankMyrrh, Gardenia, Hibiscus, Eucaliptus, and Peppermint) had similar effects, but of a smaller magnitude (i.e., 5-12%). Some EO increased medium total VFA concentration, primarily through an increase in acetate concentration. Overall, effects of EO on fermentation were subtle, and it is unlikely that these moderate in vitro effects would correspond to any substantive impact on ruminal fermentation in vivo.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalAnimal Feed Science and Technology
Volume144
Issue number1-2
DOIs
StatePublished - Jun 23 2008

Fingerprint

lauric acid
Volatile Oils
rumen fermentation
Ammonia
Fermentation
essential oils
ammonia
Monensin
monensin
sodium
Gardenia
Mentha piperita
Hibiscus
caraway
Amino Acids
deamination
Mentha piperita nothosubsp. piperita
Deamination
Silage
amino acids

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

@article{390580db19114f6798e43e95fe4e7ac8,
title = "Effects of essential oils on in vitro ruminal fermentation and ammonia release",
abstract = "Ruminal inoculum enriched with particle-associated microorganisms was collected from two lactating dairy cows fed an alfalfa hay/cereal silage/concentrate diet 1 h before feeding and used to evaluate effects of essential oils (EO) on ruminal fermentation in short-term in vitro incubations. Ruminal ammonia N was labeled with 15N and native and hydrolyzed casein were provided as sources of amino acids. Forty EO were tested at 10 and 100 mg/l final medium concentration. Monensin-Na, and sodium laurate were also incubated at 5 and 2000 mg/l, respectively. Compared with blanks (i.e., no addition of EO), sodium laurate increased medium pH and a number of EO reduced medium pH. Both sodium laurate and monensin reduced ammonia concentrations compared to the blank. Only one of the tested EO (i.e., Caraway) slightly reduced ammonia concentration, by 8{\%}, compared with the blank. Monensin and sodium laurate resulted in higher (i.e., 9-34{\%}, monensin, and 29-47{\%}, sodium laurate) 15N enrichment of ammonia N, an indication of reduced deamination of amino acids in these treatments versus the blank. Several EO (i.e., FrankMyrrh, Gardenia, Hibiscus, Eucaliptus, and Peppermint) had similar effects, but of a smaller magnitude (i.e., 5-12{\%}). Some EO increased medium total VFA concentration, primarily through an increase in acetate concentration. Overall, effects of EO on fermentation were subtle, and it is unlikely that these moderate in vitro effects would correspond to any substantive impact on ruminal fermentation in vivo.",
author = "Hristov, {Alexander Nikolov} and Ropp, {J. K.} and S. Zaman and A. Melgar",
year = "2008",
month = "6",
day = "23",
doi = "10.1016/j.anifeedsci.2007.09.034",
language = "English (US)",
volume = "144",
pages = "55--64",
journal = "Animal Feed Science and Technology",
issn = "0377-8401",
publisher = "Elsevier",
number = "1-2",

}

Effects of essential oils on in vitro ruminal fermentation and ammonia release. / Hristov, Alexander Nikolov; Ropp, J. K.; Zaman, S.; Melgar, A.

In: Animal Feed Science and Technology, Vol. 144, No. 1-2, 23.06.2008, p. 55-64.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of essential oils on in vitro ruminal fermentation and ammonia release

AU - Hristov, Alexander Nikolov

AU - Ropp, J. K.

AU - Zaman, S.

AU - Melgar, A.

PY - 2008/6/23

Y1 - 2008/6/23

N2 - Ruminal inoculum enriched with particle-associated microorganisms was collected from two lactating dairy cows fed an alfalfa hay/cereal silage/concentrate diet 1 h before feeding and used to evaluate effects of essential oils (EO) on ruminal fermentation in short-term in vitro incubations. Ruminal ammonia N was labeled with 15N and native and hydrolyzed casein were provided as sources of amino acids. Forty EO were tested at 10 and 100 mg/l final medium concentration. Monensin-Na, and sodium laurate were also incubated at 5 and 2000 mg/l, respectively. Compared with blanks (i.e., no addition of EO), sodium laurate increased medium pH and a number of EO reduced medium pH. Both sodium laurate and monensin reduced ammonia concentrations compared to the blank. Only one of the tested EO (i.e., Caraway) slightly reduced ammonia concentration, by 8%, compared with the blank. Monensin and sodium laurate resulted in higher (i.e., 9-34%, monensin, and 29-47%, sodium laurate) 15N enrichment of ammonia N, an indication of reduced deamination of amino acids in these treatments versus the blank. Several EO (i.e., FrankMyrrh, Gardenia, Hibiscus, Eucaliptus, and Peppermint) had similar effects, but of a smaller magnitude (i.e., 5-12%). Some EO increased medium total VFA concentration, primarily through an increase in acetate concentration. Overall, effects of EO on fermentation were subtle, and it is unlikely that these moderate in vitro effects would correspond to any substantive impact on ruminal fermentation in vivo.

AB - Ruminal inoculum enriched with particle-associated microorganisms was collected from two lactating dairy cows fed an alfalfa hay/cereal silage/concentrate diet 1 h before feeding and used to evaluate effects of essential oils (EO) on ruminal fermentation in short-term in vitro incubations. Ruminal ammonia N was labeled with 15N and native and hydrolyzed casein were provided as sources of amino acids. Forty EO were tested at 10 and 100 mg/l final medium concentration. Monensin-Na, and sodium laurate were also incubated at 5 and 2000 mg/l, respectively. Compared with blanks (i.e., no addition of EO), sodium laurate increased medium pH and a number of EO reduced medium pH. Both sodium laurate and monensin reduced ammonia concentrations compared to the blank. Only one of the tested EO (i.e., Caraway) slightly reduced ammonia concentration, by 8%, compared with the blank. Monensin and sodium laurate resulted in higher (i.e., 9-34%, monensin, and 29-47%, sodium laurate) 15N enrichment of ammonia N, an indication of reduced deamination of amino acids in these treatments versus the blank. Several EO (i.e., FrankMyrrh, Gardenia, Hibiscus, Eucaliptus, and Peppermint) had similar effects, but of a smaller magnitude (i.e., 5-12%). Some EO increased medium total VFA concentration, primarily through an increase in acetate concentration. Overall, effects of EO on fermentation were subtle, and it is unlikely that these moderate in vitro effects would correspond to any substantive impact on ruminal fermentation in vivo.

UR - http://www.scopus.com/inward/record.url?scp=44649120239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44649120239&partnerID=8YFLogxK

U2 - 10.1016/j.anifeedsci.2007.09.034

DO - 10.1016/j.anifeedsci.2007.09.034

M3 - Article

AN - SCOPUS:44649120239

VL - 144

SP - 55

EP - 64

JO - Animal Feed Science and Technology

JF - Animal Feed Science and Technology

SN - 0377-8401

IS - 1-2

ER -