Effects of exogenous phosphatidic acid on calcium current of ventricular myocytes

Wen Jie Zhang, Jing Yan Ge, Chun Yan Zhao, Xue Xin Zhang, Shu Zhan, Gou Gan Zhong

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To study the effects of exogenous phosphatidic acid (PA) on calcium current of ventricular myocytes. Methods: The Langendorff perfu sion method was used to isolate the ventricular myocytes of guinea pigs and the ventricular myocytes of guinea pigs were randomly divided into control groups, three dosage groups of PA 0.01, 0.1 and 1.0 μmol · L-1 and group of PTX 0. 1 mg · L-1 plus PA 1. 0 μmol · L-1 and group of H-7 2 μmol · L-1 plus PA 1. 0 μmol · L-1. The whole cell voltage clamp technique was used to record the calcium current of isolated ventricular myocytes of guinea pigs. Results: 0.01, 0.1 and 1.0 μmol · L-1 P A enhanced the calcium current from (288.70 ± 28.33) pA, (290.83 ± 25.12) pA and (279.54 ± 31.22) pA before administration to (304.10±28.31) pA (P>0.05), (349.80±30.13) pA (P<0.01) and (388.10±28.12) pA (P<0.001) after administration. The effects of PA on the calcium current was blocked by PTX (a G protein-coupled receptor blocking agent) and H-7 (a PKC blocking agent). Conclusion: PA can promote the opening of calcium chan nels of the ventricular myocytes by the pathway of membrane receptor-G protein-PKC in a dose-dependent manner.

Original languageEnglish (US)
Pages (from-to)422-424
Number of pages3
JournalJournal of Jilin University Medicine Edition
Volume32
Issue number3
StatePublished - May 1 2006

All Science Journal Classification (ASJC) codes

  • Pharmacology

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