Effects of genotype and temperature on pollen tube growth in perennial ryegrass (Lolium perenne L.)

A. Elgersma, A. G. Stephenson, A. P M den Nijs

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Low yield in seed crops of perennial ryegrass is related to low fertilization efficiency and low temperature during anthesis. To study the effect of genotype and temperature on pollen performance, we conducted greenhouse experiments at controlled temperatures. Individual florets of four genotypes that are known to differ in seed production were hand pollinated at four temperatures (14°, 18°, 22°, 26° C) both in vivo and via a semiin-vitro method involving excised florets on agar. Pollen germination and tube growth were determined with UV-fluorescence microscopy and scored in six classes at 2 h after pollination in vitro and after 0.5, 2 and 5 h in vivo. In vitro, both genotype and temperature had a significant effect on the performance of self-pollen. Pollen tube growth increased with temperature. In cross-pollinations, the pistil parent had a significant effect on pollen tube growth, and there was also a significant pistil-by-temperature interaction. In vivo, genotype and temperature significantly affected pollen performance. The genotype-by-temperature interaction was only significant 5 h after pollination. One genotype with low seed yield was pseudoself-compatible and was a relatively poor mother after cross-pollination. The effects of genotype and temperature on the growth of self-pollen might be exploited in a breeding programme.

Original languageEnglish (US)
Pages (from-to)225-230
Number of pages6
JournalSexual Plant Reproduction
Volume2
Issue number4
DOIs
StatePublished - Nov 1 1989

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

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