Effects of ginger supplementation on cell-cycle biomarkers in the normal-appearing colonic mucosa of patients at increased risk for colorectal cancer: Results from a pilot, randomized, and controlled trial

Jessica Citronberg, Roberd Bostick, Thomas Ahearn, D. Kim Turgeon, Mack T. Ruffin, Zora Djuric, Ananda Sen, Dean E. Brenner, Suzanna M. Zick

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

To estimate the effects of ginger on apoptosis, proliferation, and differentiation in the normal-appearing colonic mucosa, we randomized 20 people at increased risk for colorectal cancer to 2.0 g of ginger or placebo daily for 28 days in a pilot trial. Overall expression and distributions of Bax, Bcl-2, p21, hTERT, and MIB-1 (Ki-67) in colorectal crypts in rectal mucosa biopsies were measured using automated immunohistochemistry and quantitative image analysis. Relative to placebo, Bax expression in the ginger group decreased 15.6% (P = 0.78) in the whole crypts, 6.6% (P = 0.95) in the upper 40% (differentiation zone) of crypts, and 21.7% (P = 0.67) in the lower 60% (proliferative zone) of crypts; however, there was a 19% increase (P = 0.14) in Bax expression in the upper 40% relative to the whole crypt. While p21 and Bcl-2 expression remained relatively unchanged, hTERT expression in the whole crypts decreased by 41.2% (P = 0.05); the estimated treatment effect on hTERT expression was larger in the upper 40% of crypts (-47.9%; P = 0.04). In the ginger group, MIB-1 expression decreased in the whole crypts, upper 40% of crypts, and lower 60% of crypts by 16.9% (P = 0.39), 46.8% (P = 0.39), and 15.3% (P = 0.41), respectively. These pilot study results suggest that ginger may reduce proliferation in the normal-appearing colorectal epithelium and increase apoptosis and differentiation relative to proliferation-especially in the differentiation zone of the crypts and support a larger study to further investigate these results.

Original languageEnglish (US)
Pages (from-to)271-281
Number of pages11
JournalCancer Prevention Research
Volume6
Issue number4
DOIs
StatePublished - Apr 1 2013

Fingerprint

Ginger
Colorectal Neoplasms
Cell Cycle
Mucous Membrane
Randomized Controlled Trials
Biomarkers
Placebos
Apoptosis
Epithelium
Immunohistochemistry
Biopsy

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Citronberg, Jessica ; Bostick, Roberd ; Ahearn, Thomas ; Turgeon, D. Kim ; Ruffin, Mack T. ; Djuric, Zora ; Sen, Ananda ; Brenner, Dean E. ; Zick, Suzanna M. / Effects of ginger supplementation on cell-cycle biomarkers in the normal-appearing colonic mucosa of patients at increased risk for colorectal cancer : Results from a pilot, randomized, and controlled trial. In: Cancer Prevention Research. 2013 ; Vol. 6, No. 4. pp. 271-281.
@article{87ba2f2a148949efa868146429838b2d,
title = "Effects of ginger supplementation on cell-cycle biomarkers in the normal-appearing colonic mucosa of patients at increased risk for colorectal cancer: Results from a pilot, randomized, and controlled trial",
abstract = "To estimate the effects of ginger on apoptosis, proliferation, and differentiation in the normal-appearing colonic mucosa, we randomized 20 people at increased risk for colorectal cancer to 2.0 g of ginger or placebo daily for 28 days in a pilot trial. Overall expression and distributions of Bax, Bcl-2, p21, hTERT, and MIB-1 (Ki-67) in colorectal crypts in rectal mucosa biopsies were measured using automated immunohistochemistry and quantitative image analysis. Relative to placebo, Bax expression in the ginger group decreased 15.6{\%} (P = 0.78) in the whole crypts, 6.6{\%} (P = 0.95) in the upper 40{\%} (differentiation zone) of crypts, and 21.7{\%} (P = 0.67) in the lower 60{\%} (proliferative zone) of crypts; however, there was a 19{\%} increase (P = 0.14) in Bax expression in the upper 40{\%} relative to the whole crypt. While p21 and Bcl-2 expression remained relatively unchanged, hTERT expression in the whole crypts decreased by 41.2{\%} (P = 0.05); the estimated treatment effect on hTERT expression was larger in the upper 40{\%} of crypts (-47.9{\%}; P = 0.04). In the ginger group, MIB-1 expression decreased in the whole crypts, upper 40{\%} of crypts, and lower 60{\%} of crypts by 16.9{\%} (P = 0.39), 46.8{\%} (P = 0.39), and 15.3{\%} (P = 0.41), respectively. These pilot study results suggest that ginger may reduce proliferation in the normal-appearing colorectal epithelium and increase apoptosis and differentiation relative to proliferation-especially in the differentiation zone of the crypts and support a larger study to further investigate these results.",
author = "Jessica Citronberg and Roberd Bostick and Thomas Ahearn and Turgeon, {D. Kim} and Ruffin, {Mack T.} and Zora Djuric and Ananda Sen and Brenner, {Dean E.} and Zick, {Suzanna M.}",
year = "2013",
month = "4",
day = "1",
doi = "10.1158/1940-6207.CAPR-12-0327",
language = "English (US)",
volume = "6",
pages = "271--281",
journal = "Cancer Prevention Research",
issn = "1940-6207",
publisher = "American Association for Cancer Research Inc.",
number = "4",

}

Effects of ginger supplementation on cell-cycle biomarkers in the normal-appearing colonic mucosa of patients at increased risk for colorectal cancer : Results from a pilot, randomized, and controlled trial. / Citronberg, Jessica; Bostick, Roberd; Ahearn, Thomas; Turgeon, D. Kim; Ruffin, Mack T.; Djuric, Zora; Sen, Ananda; Brenner, Dean E.; Zick, Suzanna M.

In: Cancer Prevention Research, Vol. 6, No. 4, 01.04.2013, p. 271-281.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of ginger supplementation on cell-cycle biomarkers in the normal-appearing colonic mucosa of patients at increased risk for colorectal cancer

T2 - Results from a pilot, randomized, and controlled trial

AU - Citronberg, Jessica

AU - Bostick, Roberd

AU - Ahearn, Thomas

AU - Turgeon, D. Kim

AU - Ruffin, Mack T.

AU - Djuric, Zora

AU - Sen, Ananda

AU - Brenner, Dean E.

AU - Zick, Suzanna M.

PY - 2013/4/1

Y1 - 2013/4/1

N2 - To estimate the effects of ginger on apoptosis, proliferation, and differentiation in the normal-appearing colonic mucosa, we randomized 20 people at increased risk for colorectal cancer to 2.0 g of ginger or placebo daily for 28 days in a pilot trial. Overall expression and distributions of Bax, Bcl-2, p21, hTERT, and MIB-1 (Ki-67) in colorectal crypts in rectal mucosa biopsies were measured using automated immunohistochemistry and quantitative image analysis. Relative to placebo, Bax expression in the ginger group decreased 15.6% (P = 0.78) in the whole crypts, 6.6% (P = 0.95) in the upper 40% (differentiation zone) of crypts, and 21.7% (P = 0.67) in the lower 60% (proliferative zone) of crypts; however, there was a 19% increase (P = 0.14) in Bax expression in the upper 40% relative to the whole crypt. While p21 and Bcl-2 expression remained relatively unchanged, hTERT expression in the whole crypts decreased by 41.2% (P = 0.05); the estimated treatment effect on hTERT expression was larger in the upper 40% of crypts (-47.9%; P = 0.04). In the ginger group, MIB-1 expression decreased in the whole crypts, upper 40% of crypts, and lower 60% of crypts by 16.9% (P = 0.39), 46.8% (P = 0.39), and 15.3% (P = 0.41), respectively. These pilot study results suggest that ginger may reduce proliferation in the normal-appearing colorectal epithelium and increase apoptosis and differentiation relative to proliferation-especially in the differentiation zone of the crypts and support a larger study to further investigate these results.

AB - To estimate the effects of ginger on apoptosis, proliferation, and differentiation in the normal-appearing colonic mucosa, we randomized 20 people at increased risk for colorectal cancer to 2.0 g of ginger or placebo daily for 28 days in a pilot trial. Overall expression and distributions of Bax, Bcl-2, p21, hTERT, and MIB-1 (Ki-67) in colorectal crypts in rectal mucosa biopsies were measured using automated immunohistochemistry and quantitative image analysis. Relative to placebo, Bax expression in the ginger group decreased 15.6% (P = 0.78) in the whole crypts, 6.6% (P = 0.95) in the upper 40% (differentiation zone) of crypts, and 21.7% (P = 0.67) in the lower 60% (proliferative zone) of crypts; however, there was a 19% increase (P = 0.14) in Bax expression in the upper 40% relative to the whole crypt. While p21 and Bcl-2 expression remained relatively unchanged, hTERT expression in the whole crypts decreased by 41.2% (P = 0.05); the estimated treatment effect on hTERT expression was larger in the upper 40% of crypts (-47.9%; P = 0.04). In the ginger group, MIB-1 expression decreased in the whole crypts, upper 40% of crypts, and lower 60% of crypts by 16.9% (P = 0.39), 46.8% (P = 0.39), and 15.3% (P = 0.41), respectively. These pilot study results suggest that ginger may reduce proliferation in the normal-appearing colorectal epithelium and increase apoptosis and differentiation relative to proliferation-especially in the differentiation zone of the crypts and support a larger study to further investigate these results.

UR - http://www.scopus.com/inward/record.url?scp=84877266971&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877266971&partnerID=8YFLogxK

U2 - 10.1158/1940-6207.CAPR-12-0327

DO - 10.1158/1940-6207.CAPR-12-0327

M3 - Article

C2 - 23303903

AN - SCOPUS:84877266971

VL - 6

SP - 271

EP - 281

JO - Cancer Prevention Research

JF - Cancer Prevention Research

SN - 1940-6207

IS - 4

ER -