Effects of gradual coronary artery occlusion and exercise training on gene expression in swine heart

Marvin O. Boluyt, Georgina M. Cirrincione, Amy M. Loyd, Donna Hope Korzick, Janet L. Parker, M. Harold Laughlin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Gradual occlusion (O) of the swine left circumflex coronary artery (LCX) with an ameroid occluder results in complete O within 3 weeks, collateral vessel development, and compensatory hypertrophy. The purpose of this investigation was to determine the independent and combined effects of O and exercise training (E) on gene expression in the swine heart. Adult Yucatan miniature swine were assigned to one of the following groups (n = 6-9/group): sedentary control (S), exercise-trained (E), sedentary swine subjected to LCX occlusion (SO), and exercise-trained swine with LCX occlusion (EO). Exercise consisted of progressive treadmill running conducted 5 d/wk for 16 weeks. Gene expression was studied in myocardium isolated from the collateral-dependent left ventricle free wall (LV) and the collateral-independent septum (SEP) by RNA blotting. E and O each stimulated cardiac hypertrophy independently (p < 0.001) with no interaction. O but not E increased atrial natriuretic factor expression in the LV, but not in the SEP. E decreased the expression of β-myosin heavy chain in the LV, but not in the SEP. E retarded the expression of collagen III mRNA in SEP; but not in the LV. Exercise training and coronary artery occlusion each stimulate cardiac hypertrophy independently and induce different patterns of gene expression.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume294
Issue number1-2
DOIs
StatePublished - Jan 1 2007

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Coronary Occlusion
Gene expression
Coronary Vessels
Swine
Exercise
Gene Expression
Cardiomegaly
Exercise equipment
Myosin Heavy Chains
Atrial Natriuretic Factor
Miniature Swine
Collagen
Running
RNA
Hypertrophy
Heart Ventricles
Myocardium
Messenger RNA
Control Groups

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Boluyt, Marvin O. ; Cirrincione, Georgina M. ; Loyd, Amy M. ; Korzick, Donna Hope ; Parker, Janet L. ; Laughlin, M. Harold. / Effects of gradual coronary artery occlusion and exercise training on gene expression in swine heart. In: Molecular and Cellular Biochemistry. 2007 ; Vol. 294, No. 1-2. pp. 87-96.
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Effects of gradual coronary artery occlusion and exercise training on gene expression in swine heart. / Boluyt, Marvin O.; Cirrincione, Georgina M.; Loyd, Amy M.; Korzick, Donna Hope; Parker, Janet L.; Laughlin, M. Harold.

In: Molecular and Cellular Biochemistry, Vol. 294, No. 1-2, 01.01.2007, p. 87-96.

Research output: Contribution to journalArticle

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