Effects of histone acetylation and CpG methylation on the structure of nucleosomes

Ju Yeon Lee, Tae Hee Lee

Research output: Contribution to journalReview article

24 Scopus citations

Abstract

Nucleosomes are the fundamental packing units of the eukaryotic genome. A nucleosome core particle comprises an octameric histone core wrapped around by ∼ 147 bp DNA. Histones and DNA are targets for covalent modifications mediated by various chromatin modification enzymes. These modifications play crucial roles in various gene regulation activities. A group of common hypotheses for the mechanisms of gene regulation involves changes in the structure and structural dynamics of chromatin induced by chromatin modifications. We employed single molecule fluorescence methods to test these hypotheses by monitoring the structure and structural dynamics of nucleosomes before and after histone acetylation and DNA methylation, two of the best-conserved chromatin modifications throughout eukaryotes. Our studies revealed that these modifications induce changes in the structure and structural dynamics of nucleosomes that may contribute directly to the formation of open or repressive chromatin conformation.

Original languageEnglish (US)
Pages (from-to)974-982
Number of pages9
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1824
Issue number8
DOIs
StatePublished - Aug 1 2012

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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