Effects of impaired Ca2+ homeostasis on contraction in postinfarction myocytes

Xue Qian Zhang, Timothy I. Musch, Robert Zelis, Joseph Y. Cheung

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The significance of altered Ca2+ influx and efflux pathways on contractile abnormalities of myocytes isolated from rat hearts 3 wk after myocardial infarction (MI) was investigated by varying extracellular Ca2+ concentration ([Ca2+]0, 0.6-5.0 mM) and pacing frequency (0.1-5.0 Hz). Myocytes isolated from 3-wk MI hearts were significantly longer than those from sham-treated (Sham) hearts (125 ± 1 vs. 114 ± 1 pro, P < 0.0001). At high [Ca2+]0 and low pacing frequency, conditions that preferentially favored Ca2+ influx over efflux, Sham myocytes shortened to a greater extent than 3-wk MI myocytes. Conversely, under conditions that favored Ca2+ efflux (low [Ca2+]0 and high pacing frequency), MI myocytes shortened more than Sham myocytes. At intermediate [Ca2+]0 and pacing frequencies, differences in steady-state contraction amplitudes between Sham and MI myocytes were no longer significant. Collectively, the interpretation of these data was that Ca2+ influx and efflux pathways were subnormal in MI myocytes and that they contributed to abnormal cellular contractile behavior. Because Na+/Ca2+ exchange activity, but not whole cell Ca2+ current, was depressed in 3-wk MI rat myocytes, our results on steady-state contraction are consistent with, but not proof of, the hypothesis that depressed Na+/Ca2+ exchange accounted for abnormal contractility in MI myocytes. The effects of depressed Na+/Ca2+ exchange on MI myocyte mechanical activity were further evaluated in relaxation from caffeine-induced contractures. Because Ca2+ uptake by sarcoplasmic reticulum was inhibited by caffeine and with the assumption that intracellular Na+ and membrane potential were similar between Sham and MI myocytes, myocyte relaxation from caffeine- induced contracture can be taken as an estimate of Ca2+ extrusion by Na+/Ca2+ exchange. In MI myocytes, in which Na+/Ca2+ exchange activity was depressed, the half time of relaxation (1.54 ± 0.14 s) was significantly (P < 0.02) prolonged compared with that measured in Sham myocytes (1.10 ± 0.10 s).

Original languageEnglish (US)
Pages (from-to)943-950
Number of pages8
JournalJournal of applied physiology
Volume86
Issue number3
DOIs
StatePublished - Mar 1999

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Muscle Cells
Homeostasis
Myocardial Infarction
Caffeine
Contracture
Intracellular Membranes
Sarcoplasmic Reticulum
Membrane Potentials

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Zhang, Xue Qian ; Musch, Timothy I. ; Zelis, Robert ; Cheung, Joseph Y. / Effects of impaired Ca2+ homeostasis on contraction in postinfarction myocytes. In: Journal of applied physiology. 1999 ; Vol. 86, No. 3. pp. 943-950.
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abstract = "The significance of altered Ca2+ influx and efflux pathways on contractile abnormalities of myocytes isolated from rat hearts 3 wk after myocardial infarction (MI) was investigated by varying extracellular Ca2+ concentration ([Ca2+]0, 0.6-5.0 mM) and pacing frequency (0.1-5.0 Hz). Myocytes isolated from 3-wk MI hearts were significantly longer than those from sham-treated (Sham) hearts (125 ± 1 vs. 114 ± 1 pro, P < 0.0001). At high [Ca2+]0 and low pacing frequency, conditions that preferentially favored Ca2+ influx over efflux, Sham myocytes shortened to a greater extent than 3-wk MI myocytes. Conversely, under conditions that favored Ca2+ efflux (low [Ca2+]0 and high pacing frequency), MI myocytes shortened more than Sham myocytes. At intermediate [Ca2+]0 and pacing frequencies, differences in steady-state contraction amplitudes between Sham and MI myocytes were no longer significant. Collectively, the interpretation of these data was that Ca2+ influx and efflux pathways were subnormal in MI myocytes and that they contributed to abnormal cellular contractile behavior. Because Na+/Ca2+ exchange activity, but not whole cell Ca2+ current, was depressed in 3-wk MI rat myocytes, our results on steady-state contraction are consistent with, but not proof of, the hypothesis that depressed Na+/Ca2+ exchange accounted for abnormal contractility in MI myocytes. The effects of depressed Na+/Ca2+ exchange on MI myocyte mechanical activity were further evaluated in relaxation from caffeine-induced contractures. Because Ca2+ uptake by sarcoplasmic reticulum was inhibited by caffeine and with the assumption that intracellular Na+ and membrane potential were similar between Sham and MI myocytes, myocyte relaxation from caffeine- induced contracture can be taken as an estimate of Ca2+ extrusion by Na+/Ca2+ exchange. In MI myocytes, in which Na+/Ca2+ exchange activity was depressed, the half time of relaxation (1.54 ± 0.14 s) was significantly (P < 0.02) prolonged compared with that measured in Sham myocytes (1.10 ± 0.10 s).",
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Effects of impaired Ca2+ homeostasis on contraction in postinfarction myocytes. / Zhang, Xue Qian; Musch, Timothy I.; Zelis, Robert; Cheung, Joseph Y.

In: Journal of applied physiology, Vol. 86, No. 3, 03.1999, p. 943-950.

Research output: Contribution to journalArticle

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