Effects of insemination quantity on honey bee queen physiology

Freddie Jeanne Richard, David R. Tarpy, Christina M. Grozinger

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Mating has profound effects on the physiology and behavior of female insects, and in honey bee (Apis mellifera) queens, these changes are permanent. Queens mate with multiple males during a brief period in their early adult lives, and shortly thereafter they initiate egg-laying. Furthermore, the pheromone profiles of mated queens differ from those of virgins, and these pheromones regulate many different aspects of worker behavior and colony organization, While it is clear that mating causes dramatic changes in queens, it is unclear if mating number has more subtle effects on queen physiology or queen-worker interactions; indeed, the effect of multiple matings on female insect physiology has not been broadly addressed. Because it is not possible to control the natural mating behavior of queens, we used instrumental insemination and compared queens inseminated with semen from either a single drone (single-drone inseminated, or SDI) or 10 drones (multi-drone inseminated, or MDI). We used observation hives to monitor attraction of workers to SDI or MDI queens in colonies, and cage studies to monitor the attraction of workers to virgin, SM, and MDI queen mandibular gland extracts (the main source of queen pheromone). The chemical profiles of the mandibular glands of virgin, SDI, and MDI queens were characterized using GC-MS. Finally, we measured brain expression levels in SDI and MDI queens of a gene associated with phototaxis in worker honey bees (Amfor). Here, we demonstrate for the first time that insemination quantity significantly affects mandibular gland chemical profiles, queen-worker interactions, and brain gene expression. Further research will be necessary to elucidate the mechanistic bases for these effects. insemination volume, sperm and seminal protein quantity, and genetic diversity of the sperm may all be important factors contributing to this profound change in honey bee queen physiology, queen behavior, and social interactions in the colony.

Original languageEnglish (US)
Article numbere980
JournalPLoS One
Volume2
Issue number10
DOIs
StatePublished - Oct 3 2007

Fingerprint

queen honey bees
Honey
Insemination
Bees
Physiology
queen insects
insemination
Pheromones
physiology
Insects
Spermatozoa
Brain
drones (insects)
mandibular glands
Urticaria
Interpersonal Relations
Semen
Gene expression
Ovum
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Richard, Freddie Jeanne ; Tarpy, David R. ; Grozinger, Christina M. / Effects of insemination quantity on honey bee queen physiology. In: PLoS One. 2007 ; Vol. 2, No. 10.
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Effects of insemination quantity on honey bee queen physiology. / Richard, Freddie Jeanne; Tarpy, David R.; Grozinger, Christina M.

In: PLoS One, Vol. 2, No. 10, e980, 03.10.2007.

Research output: Contribution to journalArticle

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