Effects of intensity of acute-resistance exercise on rates of protein synthesis in moderately diabetic rats

Peter A. Farrell, Mark J. Fedele, Thomas C. Vary, Scot R. Kimball, Leonard S. Jefferson

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26 Scopus citations

Abstract

These studies determined whether increases in rates of protein synthesis observed in skeletal muscle after moderate or severe acute-resistance exercise were blunted by insulinopenia. Rats (n = 6-9 per group) were made insulin deficient by partial pancreatectomy or remained nondiabetic. Groups either remained sedentary or performed acute-resistance exercise 16 h before rates of protein synthesis were measured in vivo. Exercise required 50 repetitions of standing on the hindlimbs with either 0.6 g backpack wt/g body wt (moderate exercise) or 1.0 g backpack wt/g body wt (severe exercise). Insulin-deficient rats had a mean blood glucose concentration >15 mM and reduced insulin concentrations in the plasma. Rates of protein synthesis in gastrocnemius muscle were not different in all sedentary groups. The moderate-exercised nondiabetic group (192 ± 12 nmol phenylalanine incorporated · g muscle-1 · h-1) and moderate-exercised diabetic group (215 ± 18) had significantly (P < 0.05, ANOVA) higher rates of protein synthesis than did respective sedentary groups. In contrast, diabetic rats that performed severe-resistance exercise had rates of protein synthesis (176 ± 12) that were not different (P > 0.05) from diabetic sedentary rats (170 ± 9), whereas nondiabetic rats that performed severe exercise had higher (212 ± 24) rates compared with nondiabetic sedentary rats (178 ± 10) P < 0.05. The present data in combination with previous studies [J. D. Fluckey, T. C. Vary, L. S. Jefferson, and P. A. Farrell. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E313-E319, 1996] show that the amount of insulin required for an in vivo permissive effect of insulin on rates of protein synthesis can be quite low after moderate-intensity resistance exercise. However, severe exercise in combination with low insulin concentrations can ablate an anabolic response.

Original languageEnglish (US)
Pages (from-to)2291-2297
Number of pages7
JournalJournal of applied physiology
Volume85
Issue number6
DOIs
StatePublished - Dec 1998

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

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