Effects of intensity of acute-resistance exercise on rates of protein synthesis in moderately diabetic rats

Peter A. Farrell, Mark J. Fedele, Thomas C. Vary, Scot R. Kimball, Leonard S. Jefferson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

These studies determined whether increases in rates of protein synthesis observed in skeletal muscle after moderate or severe acute-resistance exercise were blunted by insulinopenia. Rats (n = 6-9 per group) were made insulin deficient by partial pancreatectomy or remained nondiabetic. Groups either remained sedentary or performed acute-resistance exercise 16 h before rates of protein synthesis were measured in vivo. Exercise required 50 repetitions of standing on the hindlimbs with either 0.6 g backpack wt/g body wt (moderate exercise) or 1.0 g backpack wt/g body wt (severe exercise). Insulin-deficient rats had a mean blood glucose concentration >15 mM and reduced insulin concentrations in the plasma. Rates of protein synthesis in gastrocnemius muscle were not different in all sedentary groups. The moderate-exercised nondiabetic group (192 ± 12 nmol phenylalanine incorporated · g muscle-1 · h-1) and moderate-exercised diabetic group (215 ± 18) had significantly (P < 0.05, ANOVA) higher rates of protein synthesis than did respective sedentary groups. In contrast, diabetic rats that performed severe-resistance exercise had rates of protein synthesis (176 ± 12) that were not different (P > 0.05) from diabetic sedentary rats (170 ± 9), whereas nondiabetic rats that performed severe exercise had higher (212 ± 24) rates compared with nondiabetic sedentary rats (178 ± 10) P < 0.05. The present data in combination with previous studies [J. D. Fluckey, T. C. Vary, L. S. Jefferson, and P. A. Farrell. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E313-E319, 1996] show that the amount of insulin required for an in vivo permissive effect of insulin on rates of protein synthesis can be quite low after moderate-intensity resistance exercise. However, severe exercise in combination with low insulin concentrations can ablate an anabolic response.

Original languageEnglish (US)
Pages (from-to)2291-2297
Number of pages7
JournalJournal of applied physiology
Volume85
Issue number6
DOIs
StatePublished - Dec 1998

Fingerprint

Exercise
Insulin
Proteins
Skeletal Muscle
Pancreatectomy
Hindlimb
Phenylalanine
Blood Glucose
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

@article{124999ffda0b495db68fba294e7c4abb,
title = "Effects of intensity of acute-resistance exercise on rates of protein synthesis in moderately diabetic rats",
abstract = "These studies determined whether increases in rates of protein synthesis observed in skeletal muscle after moderate or severe acute-resistance exercise were blunted by insulinopenia. Rats (n = 6-9 per group) were made insulin deficient by partial pancreatectomy or remained nondiabetic. Groups either remained sedentary or performed acute-resistance exercise 16 h before rates of protein synthesis were measured in vivo. Exercise required 50 repetitions of standing on the hindlimbs with either 0.6 g backpack wt/g body wt (moderate exercise) or 1.0 g backpack wt/g body wt (severe exercise). Insulin-deficient rats had a mean blood glucose concentration >15 mM and reduced insulin concentrations in the plasma. Rates of protein synthesis in gastrocnemius muscle were not different in all sedentary groups. The moderate-exercised nondiabetic group (192 ± 12 nmol phenylalanine incorporated · g muscle-1 · h-1) and moderate-exercised diabetic group (215 ± 18) had significantly (P < 0.05, ANOVA) higher rates of protein synthesis than did respective sedentary groups. In contrast, diabetic rats that performed severe-resistance exercise had rates of protein synthesis (176 ± 12) that were not different (P > 0.05) from diabetic sedentary rats (170 ± 9), whereas nondiabetic rats that performed severe exercise had higher (212 ± 24) rates compared with nondiabetic sedentary rats (178 ± 10) P < 0.05. The present data in combination with previous studies [J. D. Fluckey, T. C. Vary, L. S. Jefferson, and P. A. Farrell. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E313-E319, 1996] show that the amount of insulin required for an in vivo permissive effect of insulin on rates of protein synthesis can be quite low after moderate-intensity resistance exercise. However, severe exercise in combination with low insulin concentrations can ablate an anabolic response.",
author = "Farrell, {Peter A.} and Fedele, {Mark J.} and Vary, {Thomas C.} and Kimball, {Scot R.} and Jefferson, {Leonard S.}",
year = "1998",
month = "12",
doi = "10.1152/jappl.1998.85.6.2291",
language = "English (US)",
volume = "85",
pages = "2291--2297",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "6",

}

Effects of intensity of acute-resistance exercise on rates of protein synthesis in moderately diabetic rats. / Farrell, Peter A.; Fedele, Mark J.; Vary, Thomas C.; Kimball, Scot R.; Jefferson, Leonard S.

In: Journal of applied physiology, Vol. 85, No. 6, 12.1998, p. 2291-2297.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of intensity of acute-resistance exercise on rates of protein synthesis in moderately diabetic rats

AU - Farrell, Peter A.

AU - Fedele, Mark J.

AU - Vary, Thomas C.

AU - Kimball, Scot R.

AU - Jefferson, Leonard S.

PY - 1998/12

Y1 - 1998/12

N2 - These studies determined whether increases in rates of protein synthesis observed in skeletal muscle after moderate or severe acute-resistance exercise were blunted by insulinopenia. Rats (n = 6-9 per group) were made insulin deficient by partial pancreatectomy or remained nondiabetic. Groups either remained sedentary or performed acute-resistance exercise 16 h before rates of protein synthesis were measured in vivo. Exercise required 50 repetitions of standing on the hindlimbs with either 0.6 g backpack wt/g body wt (moderate exercise) or 1.0 g backpack wt/g body wt (severe exercise). Insulin-deficient rats had a mean blood glucose concentration >15 mM and reduced insulin concentrations in the plasma. Rates of protein synthesis in gastrocnemius muscle were not different in all sedentary groups. The moderate-exercised nondiabetic group (192 ± 12 nmol phenylalanine incorporated · g muscle-1 · h-1) and moderate-exercised diabetic group (215 ± 18) had significantly (P < 0.05, ANOVA) higher rates of protein synthesis than did respective sedentary groups. In contrast, diabetic rats that performed severe-resistance exercise had rates of protein synthesis (176 ± 12) that were not different (P > 0.05) from diabetic sedentary rats (170 ± 9), whereas nondiabetic rats that performed severe exercise had higher (212 ± 24) rates compared with nondiabetic sedentary rats (178 ± 10) P < 0.05. The present data in combination with previous studies [J. D. Fluckey, T. C. Vary, L. S. Jefferson, and P. A. Farrell. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E313-E319, 1996] show that the amount of insulin required for an in vivo permissive effect of insulin on rates of protein synthesis can be quite low after moderate-intensity resistance exercise. However, severe exercise in combination with low insulin concentrations can ablate an anabolic response.

AB - These studies determined whether increases in rates of protein synthesis observed in skeletal muscle after moderate or severe acute-resistance exercise were blunted by insulinopenia. Rats (n = 6-9 per group) were made insulin deficient by partial pancreatectomy or remained nondiabetic. Groups either remained sedentary or performed acute-resistance exercise 16 h before rates of protein synthesis were measured in vivo. Exercise required 50 repetitions of standing on the hindlimbs with either 0.6 g backpack wt/g body wt (moderate exercise) or 1.0 g backpack wt/g body wt (severe exercise). Insulin-deficient rats had a mean blood glucose concentration >15 mM and reduced insulin concentrations in the plasma. Rates of protein synthesis in gastrocnemius muscle were not different in all sedentary groups. The moderate-exercised nondiabetic group (192 ± 12 nmol phenylalanine incorporated · g muscle-1 · h-1) and moderate-exercised diabetic group (215 ± 18) had significantly (P < 0.05, ANOVA) higher rates of protein synthesis than did respective sedentary groups. In contrast, diabetic rats that performed severe-resistance exercise had rates of protein synthesis (176 ± 12) that were not different (P > 0.05) from diabetic sedentary rats (170 ± 9), whereas nondiabetic rats that performed severe exercise had higher (212 ± 24) rates compared with nondiabetic sedentary rats (178 ± 10) P < 0.05. The present data in combination with previous studies [J. D. Fluckey, T. C. Vary, L. S. Jefferson, and P. A. Farrell. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E313-E319, 1996] show that the amount of insulin required for an in vivo permissive effect of insulin on rates of protein synthesis can be quite low after moderate-intensity resistance exercise. However, severe exercise in combination with low insulin concentrations can ablate an anabolic response.

UR - http://www.scopus.com/inward/record.url?scp=0031768651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031768651&partnerID=8YFLogxK

U2 - 10.1152/jappl.1998.85.6.2291

DO - 10.1152/jappl.1998.85.6.2291

M3 - Article

C2 - 9843555

AN - SCOPUS:0031768651

VL - 85

SP - 2291

EP - 2297

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 6

ER -