Effects of laccase on lignin depolymerization and enzymatic hydrolysis of ensiled corn stover

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The aim of this study was to explore the synergies of laccase, a ligninolytic enzyme, with cellulose and hemicellulase amendments on ensiled corn stover. Molecular signals of lignin decomposition were observed by tetramethylammonium hydroxide thermochemolysis and gas chromatography-mass spectroscopy (TMAH-GC-MS) analysis. The significant findings suggest that ensilage might provide a platform for biological pretreatment. By partially hydrolyzing cellulose and hemicellulose into soluble sugars, ensilage facilitates laccase penetration into the lignocellulose complex to enhance lignin degradation. Downstream cellulose hydrolysis was improved 7% with increasing laccase loading rate. These results demonstrate the potential of enzymes, either directly amended or expressed by microbes during ensilage, to maximize utilization of corn stover for cellulosic biofuels and other downstream fermentations.

Original languageEnglish (US)
Pages (from-to)186-192
Number of pages7
JournalBioresource technology
Volume117
DOIs
StatePublished - Aug 1 2012

Fingerprint

Laccase
Depolymerization
Enzymatic hydrolysis
Lignin
Cellulose
lignin
cellulose
hydrolysis
maize
Enzymes
enzyme
Biofuels
biofuel
Sugars
Gas chromatography
Fermentation
fermentation
hydroxide
Hydrolysis
sugar

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

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abstract = "The aim of this study was to explore the synergies of laccase, a ligninolytic enzyme, with cellulose and hemicellulase amendments on ensiled corn stover. Molecular signals of lignin decomposition were observed by tetramethylammonium hydroxide thermochemolysis and gas chromatography-mass spectroscopy (TMAH-GC-MS) analysis. The significant findings suggest that ensilage might provide a platform for biological pretreatment. By partially hydrolyzing cellulose and hemicellulose into soluble sugars, ensilage facilitates laccase penetration into the lignocellulose complex to enhance lignin degradation. Downstream cellulose hydrolysis was improved 7{\%} with increasing laccase loading rate. These results demonstrate the potential of enzymes, either directly amended or expressed by microbes during ensilage, to maximize utilization of corn stover for cellulosic biofuels and other downstream fermentations.",
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Effects of laccase on lignin depolymerization and enzymatic hydrolysis of ensiled corn stover. / Chen, Qin; Marshall, Megan Nicole; Geib, Scott M.; Tien, Ming; Richard, Thomas Lehman.

In: Bioresource technology, Vol. 117, 01.08.2012, p. 186-192.

Research output: Contribution to journalArticle

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