Effects of moisture content and temperature on storage of Metarhizium flavoviride Conidia

D. Moore, O. K. Douro-Kpindou, Nina Ellen Jenkins, C. J. Lomer

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The effects of moisture content and temperature on the medium-term (3-4 months) storage of conidia of Metarhizium flavoviride were investigated. Conidia harvested after 24 days of culturing on rice showed greater tolerance to long storage than conidia from 12-day cultures. The moisture content of the conidia was of greatest importance; at harvest from the culture, conidial moisture contents could be >40%, while the optimal moisture content for storage was found to be 4-5%. Dried conidia stored in oil benefited from the addition of dried silica gel, as did conidia stored as powder. A range of mineral oils proved satisfactory for storage, and when dried silica gel was added to suspensions, germination levels were 79.8% after 105 days at 28-32°C. Dried conidia stored in oil maintained germination levels of up to 96 and 85% after 80 days at 10-14°C and 28-32°C respectively. Dried conidia stored as powder retained germination levels of 95% at 10-14°C, but only up to 27% at 28-32°C. In another experiment, dried conidia maintained greater than 90% germination over 128 days, with or without silica gel at 10-14°C or - 15-18°C.

Original languageEnglish (US)
Pages (from-to)51-61
Number of pages11
JournalBiocontrol Science and Technology
Volume6
Issue number1
StatePublished - Dec 1 1996

Fingerprint

Metarhizium flavoviride
Metarhizium
Fungal Spores
conidia
water content
Temperature
Germination
temperature
Silica Gel
silica gel
germination
Powders
powders
Oils
Mineral Oil
oils
mineral oil
Suspensions

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Insect Science

Cite this

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title = "Effects of moisture content and temperature on storage of Metarhizium flavoviride Conidia",
abstract = "The effects of moisture content and temperature on the medium-term (3-4 months) storage of conidia of Metarhizium flavoviride were investigated. Conidia harvested after 24 days of culturing on rice showed greater tolerance to long storage than conidia from 12-day cultures. The moisture content of the conidia was of greatest importance; at harvest from the culture, conidial moisture contents could be >40{\%}, while the optimal moisture content for storage was found to be 4-5{\%}. Dried conidia stored in oil benefited from the addition of dried silica gel, as did conidia stored as powder. A range of mineral oils proved satisfactory for storage, and when dried silica gel was added to suspensions, germination levels were 79.8{\%} after 105 days at 28-32°C. Dried conidia stored in oil maintained germination levels of up to 96 and 85{\%} after 80 days at 10-14°C and 28-32°C respectively. Dried conidia stored as powder retained germination levels of 95{\%} at 10-14°C, but only up to 27{\%} at 28-32°C. In another experiment, dried conidia maintained greater than 90{\%} germination over 128 days, with or without silica gel at 10-14°C or - 15-18°C.",
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Effects of moisture content and temperature on storage of Metarhizium flavoviride Conidia. / Moore, D.; Douro-Kpindou, O. K.; Jenkins, Nina Ellen; Lomer, C. J.

In: Biocontrol Science and Technology, Vol. 6, No. 1, 01.12.1996, p. 51-61.

Research output: Contribution to journalArticle

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AB - The effects of moisture content and temperature on the medium-term (3-4 months) storage of conidia of Metarhizium flavoviride were investigated. Conidia harvested after 24 days of culturing on rice showed greater tolerance to long storage than conidia from 12-day cultures. The moisture content of the conidia was of greatest importance; at harvest from the culture, conidial moisture contents could be >40%, while the optimal moisture content for storage was found to be 4-5%. Dried conidia stored in oil benefited from the addition of dried silica gel, as did conidia stored as powder. A range of mineral oils proved satisfactory for storage, and when dried silica gel was added to suspensions, germination levels were 79.8% after 105 days at 28-32°C. Dried conidia stored in oil maintained germination levels of up to 96 and 85% after 80 days at 10-14°C and 28-32°C respectively. Dried conidia stored as powder retained germination levels of 95% at 10-14°C, but only up to 27% at 28-32°C. In another experiment, dried conidia maintained greater than 90% germination over 128 days, with or without silica gel at 10-14°C or - 15-18°C.

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