Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells

Amy L. Mihalchik, Weiqiang Ding, Dale W. Porter, Colleen McLoughlin, Diane Schwegler-Berry, Jennifer D. Sisler, Aleksandr B. Stefaniak, Brandi N. Snyder-Talkington, Rodolfo Cruz-Silva, Mauricio Terrones, Shuji Tsuruoka, Morinobu Endo, Vincent Castranova, Yong Qian

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Nitrogen-doped multi-walled carbon nanotubes (ND-MWCNTs) are modified multi-walled carbon nanotubes (MWCNTs) with enhanced electrical properties that are used in a variety of applications, including fuel cells and sensors; however, the mode of toxic action of ND-MWCNT has yet to be fully elucidated. In the present study, we compared the interaction of ND-MWCNT or pristine MWCNT-7 with human small airway epithelial cells (SAEC) and evaluated their subsequent bioactive effects. Transmission electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and X-ray diffraction suggested the presence of N-containing defects in the lattice of the nanotube. The ND-MWCNTs were determined to be 93.3% carbon, 3.8% oxygen, and 2.9% nitrogen. A dose-response cell proliferation assay showed that low doses of ND-MWCNT (1.2 μg/ml) or MWCNT-7 (0.12 μg/ml) increased cellular proliferation, while the highest dose of 120. μg/ml of either material decreased proliferation. ND-MWCNT and MWCNT-7 appeared to interact with SAEC at 6. h and were internalized by 24 h ROS were elevated at 6 and 24 h in ND-MWCNT exposed cells, but only at 6 h in MWCNT-7 exposed cells. Significant alterations to the cell cycle were observed in SAEC exposed to either 1.2 μg/ml of ND-MWCNT or MWCNT-7 in a time and material-dependent manner, possibly suggesting potential damage or alterations to cell cycle machinery. Our results indicate that ND-MWCNT induce effects in SAEC over a time and dose-related manner which differ from MWCNT-7 Therefore, the physicochemical characteristics of the materials appear to alter their biological effects.

Original languageEnglish (US)
Pages (from-to)25-36
Number of pages12
JournalToxicology
Volume333
DOIs
StatePublished - Mar 20 2015

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Carbon Nanotubes
Nitrogen
Epithelial Cells
Cell Cycle
Cells
Cell Proliferation
Toxic Actions
Nanotubes
Photoelectron Spectroscopy
Raman Spectrum Analysis
Cell proliferation
Transmission Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Mihalchik, A. L., Ding, W., Porter, D. W., McLoughlin, C., Schwegler-Berry, D., Sisler, J. D., ... Qian, Y. (2015). Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells. Toxicology, 333, 25-36. https://doi.org/10.1016/j.tox.2015.03.008
Mihalchik, Amy L. ; Ding, Weiqiang ; Porter, Dale W. ; McLoughlin, Colleen ; Schwegler-Berry, Diane ; Sisler, Jennifer D. ; Stefaniak, Aleksandr B. ; Snyder-Talkington, Brandi N. ; Cruz-Silva, Rodolfo ; Terrones, Mauricio ; Tsuruoka, Shuji ; Endo, Morinobu ; Castranova, Vincent ; Qian, Yong. / Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells. In: Toxicology. 2015 ; Vol. 333. pp. 25-36.
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abstract = "Nitrogen-doped multi-walled carbon nanotubes (ND-MWCNTs) are modified multi-walled carbon nanotubes (MWCNTs) with enhanced electrical properties that are used in a variety of applications, including fuel cells and sensors; however, the mode of toxic action of ND-MWCNT has yet to be fully elucidated. In the present study, we compared the interaction of ND-MWCNT or pristine MWCNT-7 with human small airway epithelial cells (SAEC) and evaluated their subsequent bioactive effects. Transmission electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and X-ray diffraction suggested the presence of N-containing defects in the lattice of the nanotube. The ND-MWCNTs were determined to be 93.3{\%} carbon, 3.8{\%} oxygen, and 2.9{\%} nitrogen. A dose-response cell proliferation assay showed that low doses of ND-MWCNT (1.2 μg/ml) or MWCNT-7 (0.12 μg/ml) increased cellular proliferation, while the highest dose of 120. μg/ml of either material decreased proliferation. ND-MWCNT and MWCNT-7 appeared to interact with SAEC at 6. h and were internalized by 24 h ROS were elevated at 6 and 24 h in ND-MWCNT exposed cells, but only at 6 h in MWCNT-7 exposed cells. Significant alterations to the cell cycle were observed in SAEC exposed to either 1.2 μg/ml of ND-MWCNT or MWCNT-7 in a time and material-dependent manner, possibly suggesting potential damage or alterations to cell cycle machinery. Our results indicate that ND-MWCNT induce effects in SAEC over a time and dose-related manner which differ from MWCNT-7 Therefore, the physicochemical characteristics of the materials appear to alter their biological effects.",
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Mihalchik, AL, Ding, W, Porter, DW, McLoughlin, C, Schwegler-Berry, D, Sisler, JD, Stefaniak, AB, Snyder-Talkington, BN, Cruz-Silva, R, Terrones, M, Tsuruoka, S, Endo, M, Castranova, V & Qian, Y 2015, 'Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells', Toxicology, vol. 333, pp. 25-36. https://doi.org/10.1016/j.tox.2015.03.008

Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells. / Mihalchik, Amy L.; Ding, Weiqiang; Porter, Dale W.; McLoughlin, Colleen; Schwegler-Berry, Diane; Sisler, Jennifer D.; Stefaniak, Aleksandr B.; Snyder-Talkington, Brandi N.; Cruz-Silva, Rodolfo; Terrones, Mauricio; Tsuruoka, Shuji; Endo, Morinobu; Castranova, Vincent; Qian, Yong.

In: Toxicology, Vol. 333, 20.03.2015, p. 25-36.

Research output: Contribution to journalArticle

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T1 - Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells

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AU - Ding, Weiqiang

AU - Porter, Dale W.

AU - McLoughlin, Colleen

AU - Schwegler-Berry, Diane

AU - Sisler, Jennifer D.

AU - Stefaniak, Aleksandr B.

AU - Snyder-Talkington, Brandi N.

AU - Cruz-Silva, Rodolfo

AU - Terrones, Mauricio

AU - Tsuruoka, Shuji

AU - Endo, Morinobu

AU - Castranova, Vincent

AU - Qian, Yong

PY - 2015/3/20

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N2 - Nitrogen-doped multi-walled carbon nanotubes (ND-MWCNTs) are modified multi-walled carbon nanotubes (MWCNTs) with enhanced electrical properties that are used in a variety of applications, including fuel cells and sensors; however, the mode of toxic action of ND-MWCNT has yet to be fully elucidated. In the present study, we compared the interaction of ND-MWCNT or pristine MWCNT-7 with human small airway epithelial cells (SAEC) and evaluated their subsequent bioactive effects. Transmission electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and X-ray diffraction suggested the presence of N-containing defects in the lattice of the nanotube. The ND-MWCNTs were determined to be 93.3% carbon, 3.8% oxygen, and 2.9% nitrogen. A dose-response cell proliferation assay showed that low doses of ND-MWCNT (1.2 μg/ml) or MWCNT-7 (0.12 μg/ml) increased cellular proliferation, while the highest dose of 120. μg/ml of either material decreased proliferation. ND-MWCNT and MWCNT-7 appeared to interact with SAEC at 6. h and were internalized by 24 h ROS were elevated at 6 and 24 h in ND-MWCNT exposed cells, but only at 6 h in MWCNT-7 exposed cells. Significant alterations to the cell cycle were observed in SAEC exposed to either 1.2 μg/ml of ND-MWCNT or MWCNT-7 in a time and material-dependent manner, possibly suggesting potential damage or alterations to cell cycle machinery. Our results indicate that ND-MWCNT induce effects in SAEC over a time and dose-related manner which differ from MWCNT-7 Therefore, the physicochemical characteristics of the materials appear to alter their biological effects.

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