Effects of organic and inorganic selenium compounds on rat mammary tumor cells

Ze'Ev Ronai, Joanne K. Tillotson, Frank Traganos, Z. Darzynkiewicz, C. Clifford Conaway, Parmod Upadhyaya, Karam El‐Bayoumy

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

To explore cellular effects of potent organoselenium chemo‐preventive agents we have used a rat mammary tumor cell line. We demonstrate that 1,4‐phenylenebis(methylene) selenocyanate (p‐XSC) at a dose of 5 μM is a more potent inhibitor of DNA, RNA and protein synthesis as well as of mitochondrial transmembrane potential than its chemopreventive counterparts benzyl selenocyanate (BSC) and sodium selenite. These differences were also reflected in reduced growth rate by 24 and 48 hr. Cell‐cycle and cell‐morphology analysis revealed that higher doses of p‐XSC (10 μM) caused DNA fragmentation which was accompanied with partial loss of nuclear stainability, whereas BSC caused a noticeable change in cell‐cycle distribution and extensive micronucleation. Overall, our results point to cellular targets of selenium compounds which may mediate their chemopreventive activities in mammary tissues.

Original languageEnglish (US)
Pages (from-to)428-434
Number of pages7
JournalInternational Journal of Cancer
Volume63
Issue number3
DOIs
StatePublished - Nov 3 1995

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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