Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery

Yong Wang, He Wang, Chuan Yuan Li, Fan Yuan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Recent studies have shown that up to 90% of viral vectors could disseminate to normal organs following intratumoral infusion. The amount of dissemination might be dependent on the infusion conditions. Therefore, we investigated the effects of infusion rate, volume, and dose on transgene expression in liver and tumor tissues after intratumoral infusion of an adenoviral vector encoding luciferase. Luciferase expression was determined through bioluminescence intensity measurement. We observed that the luciferase expression in the liver was independent of the infusion rate but increased with the infusion dose, whereas the luciferase expression in the tumor was a bell-shaped function of the infusion rate. The latter observation was consistent with the distribution pattern of Evans blue - labeled albumin after its solution was infused into tumors at the same infusion rates. We also observed that the infusion volume could affect luciferase expression in the tumor but not in the liver. These observations implied that virus dissemination was determined mainly by the infusion dose, whereas the amount of transgene expression in the tumor depended on the distribution volume of viral vectors in the tumor, as well as the infusion dose.

Original languageEnglish (US)
Pages (from-to)362-366
Number of pages5
JournalMolecular cancer therapeutics
Volume5
Issue number2
DOIs
StatePublished - Feb 1 2006

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Luciferases
Viruses
Genes
Neoplasms
Transgenes
Liver
Luminescent Measurements
Evans Blue
Albumins
Observation

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

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abstract = "Recent studies have shown that up to 90{\%} of viral vectors could disseminate to normal organs following intratumoral infusion. The amount of dissemination might be dependent on the infusion conditions. Therefore, we investigated the effects of infusion rate, volume, and dose on transgene expression in liver and tumor tissues after intratumoral infusion of an adenoviral vector encoding luciferase. Luciferase expression was determined through bioluminescence intensity measurement. We observed that the luciferase expression in the liver was independent of the infusion rate but increased with the infusion dose, whereas the luciferase expression in the tumor was a bell-shaped function of the infusion rate. The latter observation was consistent with the distribution pattern of Evans blue - labeled albumin after its solution was infused into tumors at the same infusion rates. We also observed that the infusion volume could affect luciferase expression in the tumor but not in the liver. These observations implied that virus dissemination was determined mainly by the infusion dose, whereas the amount of transgene expression in the tumor depended on the distribution volume of viral vectors in the tumor, as well as the infusion dose.",
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Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery. / Wang, Yong; Wang, He; Li, Chuan Yuan; Yuan, Fan.

In: Molecular cancer therapeutics, Vol. 5, No. 2, 01.02.2006, p. 362-366.

Research output: Contribution to journalArticle

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