Effects of recombinant ovine interferon tau, placental lactogen, and growth hormone on the ovine uterus

Thomas E. Spencer, Allison Gray, Greg A. Johnson, Kristin M. Taylor, Arieh Gertler, Elisha Gootwine, Troy Ott, Fuller W. Bazer

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Studies were conducted to determine effects of intrauterine administration of recombinant ovine interferon τ (IFNτ), placental lactogen (PL), and growth hormone (GH) on endometrial function. In the first study, administration of IFNτ to cyclic ewes for one period (Days 11-15) resulted in an interestrous interval (IEI) of ~30 days, whereas administration for two periods (Days 11-15 and Days 21-25) extended the IEI to greater than 50 days. Administration of IFNτ from Days 11 to 15 and of PL or GH from Days 21 to 25 failed to extend the IEI more than for IFNτ alone. In the second study, effects of IFNτ, PL, and GH on endometrial differentiation and function were determined in ovariectomized ewes receiving ovarian steroid replacement therapy. Endometrial expression of mRNAs for estrogen receptor (ER), progesterone receptor (PR), and oxytocin receptor (OTR) were not affected by PL or GH treatment; however, uterine milk protein mRNA levels and stratum spongiosum gland density were increased by both PL and GH treatments. Collectively, results indicated that 1) PL and GH do not regulate endometrial PR, ER, and OTR expression or affect corpus luteum life span; 2) down- regulation of epithelial PR expression is requisite for progesterone induction of secretory gene expression in uterine glandular epithelium; 3) effects of PL and GH on endometrial function require IFNτ; and 4) PL and GH regulate endometrial gland proliferation and perhaps differentiated function.

Original languageEnglish (US)
Pages (from-to)1409-1418
Number of pages10
JournalBiology of Reproduction
Volume61
Issue number6
DOIs
StatePublished - Jan 1 1999

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Placental Lactogen
Growth Hormone
Uterus
Sheep
Interferons
Progesterone Receptors
Oxytocin Receptors
Estrogen Receptors
Messenger RNA
trophoblastin
Corpus Luteum
Progesterone
Down-Regulation
Epithelium
Steroids
Gene Expression

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Spencer, T. E., Gray, A., Johnson, G. A., Taylor, K. M., Gertler, A., Gootwine, E., ... Bazer, F. W. (1999). Effects of recombinant ovine interferon tau, placental lactogen, and growth hormone on the ovine uterus. Biology of Reproduction, 61(6), 1409-1418. https://doi.org/10.1095/biolreprod61.6.1409
Spencer, Thomas E. ; Gray, Allison ; Johnson, Greg A. ; Taylor, Kristin M. ; Gertler, Arieh ; Gootwine, Elisha ; Ott, Troy ; Bazer, Fuller W. / Effects of recombinant ovine interferon tau, placental lactogen, and growth hormone on the ovine uterus. In: Biology of Reproduction. 1999 ; Vol. 61, No. 6. pp. 1409-1418.
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abstract = "Studies were conducted to determine effects of intrauterine administration of recombinant ovine interferon τ (IFNτ), placental lactogen (PL), and growth hormone (GH) on endometrial function. In the first study, administration of IFNτ to cyclic ewes for one period (Days 11-15) resulted in an interestrous interval (IEI) of ~30 days, whereas administration for two periods (Days 11-15 and Days 21-25) extended the IEI to greater than 50 days. Administration of IFNτ from Days 11 to 15 and of PL or GH from Days 21 to 25 failed to extend the IEI more than for IFNτ alone. In the second study, effects of IFNτ, PL, and GH on endometrial differentiation and function were determined in ovariectomized ewes receiving ovarian steroid replacement therapy. Endometrial expression of mRNAs for estrogen receptor (ER), progesterone receptor (PR), and oxytocin receptor (OTR) were not affected by PL or GH treatment; however, uterine milk protein mRNA levels and stratum spongiosum gland density were increased by both PL and GH treatments. Collectively, results indicated that 1) PL and GH do not regulate endometrial PR, ER, and OTR expression or affect corpus luteum life span; 2) down- regulation of epithelial PR expression is requisite for progesterone induction of secretory gene expression in uterine glandular epithelium; 3) effects of PL and GH on endometrial function require IFNτ; and 4) PL and GH regulate endometrial gland proliferation and perhaps differentiated function.",
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Spencer, TE, Gray, A, Johnson, GA, Taylor, KM, Gertler, A, Gootwine, E, Ott, T & Bazer, FW 1999, 'Effects of recombinant ovine interferon tau, placental lactogen, and growth hormone on the ovine uterus', Biology of Reproduction, vol. 61, no. 6, pp. 1409-1418. https://doi.org/10.1095/biolreprod61.6.1409

Effects of recombinant ovine interferon tau, placental lactogen, and growth hormone on the ovine uterus. / Spencer, Thomas E.; Gray, Allison; Johnson, Greg A.; Taylor, Kristin M.; Gertler, Arieh; Gootwine, Elisha; Ott, Troy; Bazer, Fuller W.

In: Biology of Reproduction, Vol. 61, No. 6, 01.01.1999, p. 1409-1418.

Research output: Contribution to journalArticle

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AU - Spencer, Thomas E.

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