Effects of streptozotocin-induced diabetes and insulin treatment on substance P of the rat arterial wall

Ronald Albert Markle, Dawn K. Walker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Substance P (SP) is present in perivascular nerves throughout the mammalian vasculature. Reports of diminished SP levels in nerve and gastrointestinal tissues of diabetic rats led us to examine SP-like immunoreactivity (SP-LI) in large arteries by RIA. Six weeks after inducing diabetes with streptozotocin (STZ), SP-LI was measured in the thoracic aorta, abdominal aorta, and the proximal superior mesenteric artery. In diabetics we measured a doubling (P < 0.01) of SP-LI in all three artery wall preparations. This finding was verified in a second experiment which included a subset of diabetics treated daily with insulin for the sixth week of the holding period. Again, we measured a two-fold or greater increase of SP-LI (P < 0.01-0.05) in arteries from the diabetics and found that insulin treatment significantly reduced SP-LI (P < 0.05). In contrast to reports of diminished SP content in other tissues of diabetic rats, our findings demonstrate that the artery wall experiences at least a twofold increase of SP-LI in the diabetic state. Furthermore, this elevation of SP-LI is reduced by insulin. We speculate that these changes of arterial wall SP-LI may contribute to altered regulation of the vascular system in the diabetic state.

Original languageEnglish (US)
Pages (from-to)1123-1129
Number of pages7
JournalLife Sciences
Volume58
Issue number14
DOIs
StatePublished - Mar 1 1996

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Experimental Diabetes Mellitus
Substance P
Streptozocin
Medical problems
Rats
Insulin
Arteries
Tissue
Nerve Tissue
Superior Mesenteric Artery
Abdominal Aorta
Thoracic Aorta
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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abstract = "Substance P (SP) is present in perivascular nerves throughout the mammalian vasculature. Reports of diminished SP levels in nerve and gastrointestinal tissues of diabetic rats led us to examine SP-like immunoreactivity (SP-LI) in large arteries by RIA. Six weeks after inducing diabetes with streptozotocin (STZ), SP-LI was measured in the thoracic aorta, abdominal aorta, and the proximal superior mesenteric artery. In diabetics we measured a doubling (P < 0.01) of SP-LI in all three artery wall preparations. This finding was verified in a second experiment which included a subset of diabetics treated daily with insulin for the sixth week of the holding period. Again, we measured a two-fold or greater increase of SP-LI (P < 0.01-0.05) in arteries from the diabetics and found that insulin treatment significantly reduced SP-LI (P < 0.05). In contrast to reports of diminished SP content in other tissues of diabetic rats, our findings demonstrate that the artery wall experiences at least a twofold increase of SP-LI in the diabetic state. Furthermore, this elevation of SP-LI is reduced by insulin. We speculate that these changes of arterial wall SP-LI may contribute to altered regulation of the vascular system in the diabetic state.",
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Effects of streptozotocin-induced diabetes and insulin treatment on substance P of the rat arterial wall. / Markle, Ronald Albert; Walker, Dawn K.

In: Life Sciences, Vol. 58, No. 14, 01.03.1996, p. 1123-1129.

Research output: Contribution to journalArticle

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