Effects of surfactant protein A and surfactant lipids on lymphocyte proliferation in vitro

S. G. Kremlev, T. M. Umstead, David Phelps

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We studied the effects of dipalmitoyl L-α-phosphatidylcholine (DPPC), Survanta, surfactant protein A (SP-A), and mixtures of these substances on mitogen-induced lymphocyte proliferation using concanavalin A as a mitogen. A concentration-dependent suppression of proliferation was observed with 50- 250 μg/ml of DPPC or Survanta. However, when SP-A was added to cultures, proliferation was stimulated. The inhibitory effects of DPPC and Survanta were altered in mixtures that contained SP-A. When added to 50 μg/ml of Survanta, SP-A reversed the inhibitory influence of Survanta and caused increased proliferation. These findings suggest that surfactant phospholipids cause a suppression of mitogen-induced lymphocyte proliferation, which is reversed somewhat by addition of SP-A. We hypothesize that immune cell function in the lung varies with changes in the relative amounts of surfactant components. Changes in surfactant composition may occur during pulmonary inflammation or infection or with surfactant replacement therapy and may influence immune and inflammatory processes in the lung.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume267
Issue number4 11-4
StatePublished - Jan 1 1994

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Pulmonary Surfactant-Associated Protein A
Surface-Active Agents
1,2-Dipalmitoylphosphatidylcholine
Lymphocytes
Lipids
Mitogens
Lung
Concanavalin A
Phospholipids
Pneumonia
In Vitro Techniques
beractant
Infection

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

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Effects of surfactant protein A and surfactant lipids on lymphocyte proliferation in vitro. / Kremlev, S. G.; Umstead, T. M.; Phelps, David.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 267, No. 4 11-4, 01.01.1994.

Research output: Contribution to journalArticle

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