Effects of the estrous cycle and early pregnancy on uterine expression of Mx protein in sheep (Ovis aries)

Troy Ott, Jianyun Yin, Anne A. Wiley, Hun Taek Kim, Behzad Gerami-Naini, Thomas E. Spencer, Frank F. Bartol, Robert C. Burghardt, Fuller W. Bazer

Research output: Contribution to journalArticle

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Abstract

Conceptuses of ruminant ungulates produce large amounts of a type I interferon, interferon-tau (IFNτ), which is the signal for maternal recognition of pregnancy. Induction of cellular Mx proteins is an important component of the response to type I interferon in the immune system, but Mx regulation and function have not been studied in the uterus. This study examined temporal and spatial alterations in ovine uterine Mx expression during the cycle and early pregnancy using immunohistochemistry, in situ hybridization, and Northern and slot-blot analysis. Sheep uterine endometrium expressed a single ~2.5-kilobase Mx mRNA transcript that was detectable at all stages of the estrous cycle and early pregnancy examined. In cyclic ewes, mRNA abundance in endometrium increased from Day 1 to peak levels at Day 13 and then declined to Day 15. In pregnant ewes, steady-state levels of Mx mRNA were first detected above the level in cyclic ewes at Day 13 postmating, were greater than 10-fold higher at Day 15, and remained elevated at Day 19. Expression of Mx mRNA in the myometrium did not change during the estrous cycle but increased ~23-fold between Days 11 and 15 of pregnancy. Immunohistochemical and in situ hybridization analysis revealed a similar temporal pattern of Mx expression. In cyclic ewes, Mx protein and mRNA were initially localized to the luminal epithelium at Days 1 and 3, increased from Days 5 to 13, especially in the shallow uterine glands, and then declined at Day 15. Pregnancy resulted in up-regulation of Mx expression in the luminal and glandular epithelium, stroma, and myometrium. Punctate Mx immunostaining and Mx mRNA concentrations were greatest when progesterone production was maximal during the estrous cycle and were strongly upregulated by the conceptus across the entire uterine wall. It is suggested that a cascade of induction of Mx gene expression proceeds from the luminal epithelium to the outer longitudinal myometrium and that transcriptional activation of the promoter may involve both soluble cytokines (i.e., IFNτ) and steroid hormones (i.e., progesterone).

Original languageEnglish (US)
Pages (from-to)784-794
Number of pages11
JournalBiology of reproduction
Volume59
Issue number4
DOIs
StatePublished - Oct 1 1998

Fingerprint

Myxovirus Resistance Proteins
Domestic Sheep
Estrous Cycle
Sheep
Pregnancy
Messenger RNA
Myometrium
Interferon Type I
Epithelium
Endometrium
In Situ Hybridization
Progesterone
Ruminants
Northern Blotting
Transcriptional Activation
Uterus
Immune System
Up-Regulation
Immunohistochemistry
Steroids

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Ott, Troy ; Yin, Jianyun ; Wiley, Anne A. ; Kim, Hun Taek ; Gerami-Naini, Behzad ; Spencer, Thomas E. ; Bartol, Frank F. ; Burghardt, Robert C. ; Bazer, Fuller W. / Effects of the estrous cycle and early pregnancy on uterine expression of Mx protein in sheep (Ovis aries). In: Biology of reproduction. 1998 ; Vol. 59, No. 4. pp. 784-794.
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abstract = "Conceptuses of ruminant ungulates produce large amounts of a type I interferon, interferon-tau (IFNτ), which is the signal for maternal recognition of pregnancy. Induction of cellular Mx proteins is an important component of the response to type I interferon in the immune system, but Mx regulation and function have not been studied in the uterus. This study examined temporal and spatial alterations in ovine uterine Mx expression during the cycle and early pregnancy using immunohistochemistry, in situ hybridization, and Northern and slot-blot analysis. Sheep uterine endometrium expressed a single ~2.5-kilobase Mx mRNA transcript that was detectable at all stages of the estrous cycle and early pregnancy examined. In cyclic ewes, mRNA abundance in endometrium increased from Day 1 to peak levels at Day 13 and then declined to Day 15. In pregnant ewes, steady-state levels of Mx mRNA were first detected above the level in cyclic ewes at Day 13 postmating, were greater than 10-fold higher at Day 15, and remained elevated at Day 19. Expression of Mx mRNA in the myometrium did not change during the estrous cycle but increased ~23-fold between Days 11 and 15 of pregnancy. Immunohistochemical and in situ hybridization analysis revealed a similar temporal pattern of Mx expression. In cyclic ewes, Mx protein and mRNA were initially localized to the luminal epithelium at Days 1 and 3, increased from Days 5 to 13, especially in the shallow uterine glands, and then declined at Day 15. Pregnancy resulted in up-regulation of Mx expression in the luminal and glandular epithelium, stroma, and myometrium. Punctate Mx immunostaining and Mx mRNA concentrations were greatest when progesterone production was maximal during the estrous cycle and were strongly upregulated by the conceptus across the entire uterine wall. It is suggested that a cascade of induction of Mx gene expression proceeds from the luminal epithelium to the outer longitudinal myometrium and that transcriptional activation of the promoter may involve both soluble cytokines (i.e., IFNτ) and steroid hormones (i.e., progesterone).",
author = "Troy Ott and Jianyun Yin and Wiley, {Anne A.} and Kim, {Hun Taek} and Behzad Gerami-Naini and Spencer, {Thomas E.} and Bartol, {Frank F.} and Burghardt, {Robert C.} and Bazer, {Fuller W.}",
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Ott, T, Yin, J, Wiley, AA, Kim, HT, Gerami-Naini, B, Spencer, TE, Bartol, FF, Burghardt, RC & Bazer, FW 1998, 'Effects of the estrous cycle and early pregnancy on uterine expression of Mx protein in sheep (Ovis aries)', Biology of reproduction, vol. 59, no. 4, pp. 784-794. https://doi.org/10.1095/biolreprod59.4.784

Effects of the estrous cycle and early pregnancy on uterine expression of Mx protein in sheep (Ovis aries). / Ott, Troy; Yin, Jianyun; Wiley, Anne A.; Kim, Hun Taek; Gerami-Naini, Behzad; Spencer, Thomas E.; Bartol, Frank F.; Burghardt, Robert C.; Bazer, Fuller W.

In: Biology of reproduction, Vol. 59, No. 4, 01.10.1998, p. 784-794.

Research output: Contribution to journalArticle

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T1 - Effects of the estrous cycle and early pregnancy on uterine expression of Mx protein in sheep (Ovis aries)

AU - Ott, Troy

AU - Yin, Jianyun

AU - Wiley, Anne A.

AU - Kim, Hun Taek

AU - Gerami-Naini, Behzad

AU - Spencer, Thomas E.

AU - Bartol, Frank F.

AU - Burghardt, Robert C.

AU - Bazer, Fuller W.

PY - 1998/10/1

Y1 - 1998/10/1

N2 - Conceptuses of ruminant ungulates produce large amounts of a type I interferon, interferon-tau (IFNτ), which is the signal for maternal recognition of pregnancy. Induction of cellular Mx proteins is an important component of the response to type I interferon in the immune system, but Mx regulation and function have not been studied in the uterus. This study examined temporal and spatial alterations in ovine uterine Mx expression during the cycle and early pregnancy using immunohistochemistry, in situ hybridization, and Northern and slot-blot analysis. Sheep uterine endometrium expressed a single ~2.5-kilobase Mx mRNA transcript that was detectable at all stages of the estrous cycle and early pregnancy examined. In cyclic ewes, mRNA abundance in endometrium increased from Day 1 to peak levels at Day 13 and then declined to Day 15. In pregnant ewes, steady-state levels of Mx mRNA were first detected above the level in cyclic ewes at Day 13 postmating, were greater than 10-fold higher at Day 15, and remained elevated at Day 19. Expression of Mx mRNA in the myometrium did not change during the estrous cycle but increased ~23-fold between Days 11 and 15 of pregnancy. Immunohistochemical and in situ hybridization analysis revealed a similar temporal pattern of Mx expression. In cyclic ewes, Mx protein and mRNA were initially localized to the luminal epithelium at Days 1 and 3, increased from Days 5 to 13, especially in the shallow uterine glands, and then declined at Day 15. Pregnancy resulted in up-regulation of Mx expression in the luminal and glandular epithelium, stroma, and myometrium. Punctate Mx immunostaining and Mx mRNA concentrations were greatest when progesterone production was maximal during the estrous cycle and were strongly upregulated by the conceptus across the entire uterine wall. It is suggested that a cascade of induction of Mx gene expression proceeds from the luminal epithelium to the outer longitudinal myometrium and that transcriptional activation of the promoter may involve both soluble cytokines (i.e., IFNτ) and steroid hormones (i.e., progesterone).

AB - Conceptuses of ruminant ungulates produce large amounts of a type I interferon, interferon-tau (IFNτ), which is the signal for maternal recognition of pregnancy. Induction of cellular Mx proteins is an important component of the response to type I interferon in the immune system, but Mx regulation and function have not been studied in the uterus. This study examined temporal and spatial alterations in ovine uterine Mx expression during the cycle and early pregnancy using immunohistochemistry, in situ hybridization, and Northern and slot-blot analysis. Sheep uterine endometrium expressed a single ~2.5-kilobase Mx mRNA transcript that was detectable at all stages of the estrous cycle and early pregnancy examined. In cyclic ewes, mRNA abundance in endometrium increased from Day 1 to peak levels at Day 13 and then declined to Day 15. In pregnant ewes, steady-state levels of Mx mRNA were first detected above the level in cyclic ewes at Day 13 postmating, were greater than 10-fold higher at Day 15, and remained elevated at Day 19. Expression of Mx mRNA in the myometrium did not change during the estrous cycle but increased ~23-fold between Days 11 and 15 of pregnancy. Immunohistochemical and in situ hybridization analysis revealed a similar temporal pattern of Mx expression. In cyclic ewes, Mx protein and mRNA were initially localized to the luminal epithelium at Days 1 and 3, increased from Days 5 to 13, especially in the shallow uterine glands, and then declined at Day 15. Pregnancy resulted in up-regulation of Mx expression in the luminal and glandular epithelium, stroma, and myometrium. Punctate Mx immunostaining and Mx mRNA concentrations were greatest when progesterone production was maximal during the estrous cycle and were strongly upregulated by the conceptus across the entire uterine wall. It is suggested that a cascade of induction of Mx gene expression proceeds from the luminal epithelium to the outer longitudinal myometrium and that transcriptional activation of the promoter may involve both soluble cytokines (i.e., IFNτ) and steroid hormones (i.e., progesterone).

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