Effects of timing and dose of vitamin A on tissue retinol concentrations and antibody production in the previously vitamin A-depleted rats

A. M.G. Pasatiempo, M. Abaza, C. E. Taylor, A. C. Ross

Research output: Contribution to journalArticle

12 Scopus citations


The effect of retinol repletion in previously vitamin A-depleted Lewis rats on antibody production to pneumococcal polysaccharide (SSS-III) was studied. When vitamin A-depleted rats were given either 0.35 μmol (0.1 mg) or 5.2 μmol (1.5 mg) retinol, plasma retinol became normal within 8 h. Liver and lymphoid-organ retinol concentrations were normalized by 1 d after repletion with 5.2 μmol but not 0.35 μmol retinol. Antibody production to SSS-III was compared after administering 5.2 μmol retinol either as a divided dose (half given 4 d before and half given on the day of immunization) or as a single dose concurrent with immunization. Vitamin A-depleted rats produced very little SSS-III-specific antibody. The divided dose of retinol consistently restored anti-SSS-III production whereas the single concurrent dose was less effective despite equal effect on tissue retinol concentrations. Interestingly, normalization of plasma retinol was not always a good predictor of the immune response to pneumococcal polysaccharide.

Original languageEnglish (US)
Pages (from-to)443-451
Number of pages9
JournalAmerican Journal of Clinical Nutrition
Issue number2
Publication statusPublished - Jan 1 1992


All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this