Efficacy of sulfuric acid scarification and disinfectant treatments in eliminating Escherichia coli O157:H7 from alfalfa seeds prior to sprouting

S. Pandrangi, M. W. Elwell, R. C. Anantheswaran, L. F. LaBorde

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

E. coli O157:H7 reduction on inoculated alfalfa seeds was investigated using acid scarification treatments with or without subsequent application of sanitizers. Scarification with 0.1 to 2N H2SO4 for 2.5 to 45 min did not affect (p ≤ 0.05) seed viability. E. coli O157:H7 was reduced by 2.1 to 5.0 logs after treating with 0.1 to 2N H2SO4 for 5 to 20 min. Combined scarification (0.5N H2SO4) and H2O2 or CH3COOH treatments enhanced microbial destruction by less than 1 log compared to sanitizer alone. Chlorine, Na 2CO3, or Na3PO4 treatments preceded by scarification did not significantly increase microbial destruction compared to sanitizer alone. Appreciable reductions in seed germination were only observed with chlorine treatments.

Original languageEnglish (US)
Pages (from-to)613-617
Number of pages5
JournalJournal of Food Science
Volume68
Issue number2
StatePublished - Mar 2003

All Science Journal Classification (ASJC) codes

  • Food Science

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