Efficient and reliable transfection of mouse erythroleukemia cells using cationic lipids

Laura Elnitski, Ross Hardison

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Preliminary studies of cis-regulatory elements are frequently performed in transiently transfected cells before further analysis in stably transfected cell lines and transgenic mice. However, not all cells are readily transfectable by routine means. For instance, mouse erythroleukemia (MEL) cells have been a valuable model system for studies of their endogenous globin genes, but introduction of DNA using common transfection methods such as electroporation has been very inefficient. This has allowed studies of stably transfected cells, after selection for the rare transfection events, but transient transfection analysis has been problematic. This report describes an efficient and reliable method for transient transfection of MEL cells using commercially available cationic lipids. (C) 1999 Academic Press.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalBlood Cells, Molecules, and Diseases
Volume25
Issue number5-6
DOIs
StatePublished - Jun 1999

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Hematology
  • Cell Biology

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