TY - JOUR
T1 - Efficient excitation energy transfer among multiple dyes in polystyrene microspheres
AU - Roberts, Daniel V.
AU - Wittmershaus, Bruce P.
AU - Zhang, Yu Zhong
AU - Swan, Sharon
AU - Klinosky, Michael P.
N1 - Funding Information:
Our appreciation to Molecular Probes, Inc. for providing samples for this research. We would like to thank many people from Molecular Probes who contributed their time and ideas in valuable discussions concerning our results. This work was supported by DVR from a research grant and by BPW from start-up funds through the Pennsylvania State University: Erie, The Behrend College.
PY - 1998/11/30
Y1 - 1998/11/30
N2 - Our comparison of fluorescence excitation and absorption spectra from 40 nm polystyrene spheres containing six different dyes illustrates efficient excitation energy transfer among the dyes. The six-dye spheres collective absorption covers the entire visible region. The five dyes with the highest first excited state energy levels transfer their excitations to the sixth dye of the lowest energy level. The average excitation transfer efficiency to the sixth dye is 95% over most of the visible spectrum. As a result of this transfer, the spheres have a fluorescence maximum at 718 nm, the peak of emission from the lowest energy level dye. Each sphere functions as an artificial light-harvesting network. They have practical applications as fluorescent labels for biological systems and act as an efficient, broadly absorbing wavelength converter.
AB - Our comparison of fluorescence excitation and absorption spectra from 40 nm polystyrene spheres containing six different dyes illustrates efficient excitation energy transfer among the dyes. The six-dye spheres collective absorption covers the entire visible region. The five dyes with the highest first excited state energy levels transfer their excitations to the sixth dye of the lowest energy level. The average excitation transfer efficiency to the sixth dye is 95% over most of the visible spectrum. As a result of this transfer, the spheres have a fluorescence maximum at 718 nm, the peak of emission from the lowest energy level dye. Each sphere functions as an artificial light-harvesting network. They have practical applications as fluorescent labels for biological systems and act as an efficient, broadly absorbing wavelength converter.
UR - http://www.scopus.com/inward/record.url?scp=0032205760&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032205760&partnerID=8YFLogxK
U2 - 10.1016/S0022-2313(98)00044-1
DO - 10.1016/S0022-2313(98)00044-1
M3 - Article
AN - SCOPUS:0032205760
VL - 79
SP - 225
EP - 231
JO - Journal of Luminescence
JF - Journal of Luminescence
SN - 0022-2313
IS - 4
ER -