Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae

Philip Stewart, Ross E. Whitwam, Philip J. Kersten, Daniel Cullen, Ming Tien

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

A manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium was efficiently expressed in Aspergillus oryzae. Expression was achieved by fusing the mature eDNA of mnp1 with the A. oryzae Taka amylase promoter and secretion signal. The 3' untranslated region of the glucoamylase gene of Aspergillus awamori provided the terminator. The recombinant protein (rMnP) was secreted in an active form, permitting rapid detection and purification. Physical and kinetic properties of rMnP were similar to those of the native protein. The A. oryzae expression system is well suited for both mechanistic and site-directed mutagenesis studies.

Original languageEnglish (US)
Pages (from-to)860-864
Number of pages5
JournalApplied and environmental microbiology
Volume62
Issue number3
StatePublished - Mar 1 1996

Fingerprint

manganese peroxidase
Phanerochaete
Phanerochaete chrysosporium
Aspergillus oryzae
manganese
Recombinant Proteins
recombinant proteins
protein
gene
Aspergillus awamori
Genes
Glucan 1,4-alpha-Glucosidase
glucan 1,4-alpha-glucosidase
genes
site-directed mutagenesis
3' Untranslated Regions
3' untranslated regions
Aspergillus
Amylases
Site-Directed Mutagenesis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Stewart, Philip ; Whitwam, Ross E. ; Kersten, Philip J. ; Cullen, Daniel ; Tien, Ming. / Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. In: Applied and environmental microbiology. 1996 ; Vol. 62, No. 3. pp. 860-864.
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Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. / Stewart, Philip; Whitwam, Ross E.; Kersten, Philip J.; Cullen, Daniel; Tien, Ming.

In: Applied and environmental microbiology, Vol. 62, No. 3, 01.03.1996, p. 860-864.

Research output: Contribution to journalArticle

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