Efficient nonviral cutaneous transfection

Jill Glasspool-Malone, Stella Somiari, Joseph J. Drabick, Robert W. Malone

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Preclinical in vivo rodent, porcine, and primate experiments aimed at enhancing nonviral transgene delivery to skin have been performed. These investigations have identified a compound (aurintricarboxylic acid or ATA) that enhances transfection activity of "naked" plasmid and pulsed electrical fields (electroporation or EP) that synergistically boosts transgene expression to an average of 115-fold more than that observed with free DNA (P < 0.00009). When plasmid is intradermally injected with or without ATA, the transfected cells are typically restricted to the epidermis. However, when electroporation is added after the same injection, larger numbers of adipocytes and fibroblasts and numerous dendritic-like cells within the dermis and subdermal tissues are transfected. This advance creates new opportunities for cutaneous gene therapy and nucleic acid vaccine development.

Original languageEnglish (US)
Pages (from-to)140-146
Number of pages7
JournalMolecular Therapy
Volume2
Issue number2
DOIs
StatePublished - Aug 2000

Fingerprint

Electroporation
Transgenes
Transfection
Plasmids
Aurintricarboxylic Acid
Skin
DNA Vaccines
Dermis
Adipocytes
Epidermis
Genetic Therapy
Dendritic Cells
Primates
Rodentia
Swine
Fibroblasts
Injections
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

Cite this

Glasspool-Malone, J., Somiari, S., Drabick, J. J., & Malone, R. W. (2000). Efficient nonviral cutaneous transfection. Molecular Therapy, 2(2), 140-146. https://doi.org/10.1006/mthe.2000.0107
Glasspool-Malone, Jill ; Somiari, Stella ; Drabick, Joseph J. ; Malone, Robert W. / Efficient nonviral cutaneous transfection. In: Molecular Therapy. 2000 ; Vol. 2, No. 2. pp. 140-146.
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Glasspool-Malone, J, Somiari, S, Drabick, JJ & Malone, RW 2000, 'Efficient nonviral cutaneous transfection', Molecular Therapy, vol. 2, no. 2, pp. 140-146. https://doi.org/10.1006/mthe.2000.0107

Efficient nonviral cutaneous transfection. / Glasspool-Malone, Jill; Somiari, Stella; Drabick, Joseph J.; Malone, Robert W.

In: Molecular Therapy, Vol. 2, No. 2, 08.2000, p. 140-146.

Research output: Contribution to journalArticle

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