Eight polymorphic microsatellite loci for the Indo-Pacific-wide zoanthid, Zoanthus sansibaricus

Drew C. Wham, Margaux Carmichael, James D. Reimer, Todd C. LaJeunesse

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Next generation sequencing allows rapid development of genetic markers for investigating the ecology and evolution of non-model organisms. In the present study we employed 454 sequencing and high through-put screening to ultimately generate eight highly polymorphic microsatellites for Zoanthus sansibaricus, a common colonial anemone in the order Zoantharia that is widely distributed throughout the Indo-Pacific. Each locus was screened against cultured Symbiodinium as well as on individuals harboring distantly related symbiont species to discount contamination by DNA from their obligate dinoflagellate symbionts. The range of Z. sansibaricus extends across the tropical and subtropical Indian and Pacific oceans. Therefore, these population genetic markers will allow examination of dispersal and gene flow in this species across large tropical and subtropical oceanic ranges.

Original languageEnglish (US)
Pages (from-to)247-250
Number of pages4
JournalMarine Biodiversity
Volume43
Issue number3
DOIs
StatePublished - Sep 1 2013

Fingerprint

genetic marker
symbiont
symbionts
microsatellite repeats
Symbiodinium
loci
genetic markers
Indian Ocean
Pacific Ocean
dinoflagellate
population genetics
gene flow
ecology
screening
DNA
organisms
ocean
Zoantharia
Miozoa
organism

All Science Journal Classification (ASJC) codes

  • Oceanography
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Wham, Drew C. ; Carmichael, Margaux ; Reimer, James D. ; LaJeunesse, Todd C. / Eight polymorphic microsatellite loci for the Indo-Pacific-wide zoanthid, Zoanthus sansibaricus. In: Marine Biodiversity. 2013 ; Vol. 43, No. 3. pp. 247-250.
@article{8423feaf2b354506a2bcbf5a8635e095,
title = "Eight polymorphic microsatellite loci for the Indo-Pacific-wide zoanthid, Zoanthus sansibaricus",
abstract = "Next generation sequencing allows rapid development of genetic markers for investigating the ecology and evolution of non-model organisms. In the present study we employed 454 sequencing and high through-put screening to ultimately generate eight highly polymorphic microsatellites for Zoanthus sansibaricus, a common colonial anemone in the order Zoantharia that is widely distributed throughout the Indo-Pacific. Each locus was screened against cultured Symbiodinium as well as on individuals harboring distantly related symbiont species to discount contamination by DNA from their obligate dinoflagellate symbionts. The range of Z. sansibaricus extends across the tropical and subtropical Indian and Pacific oceans. Therefore, these population genetic markers will allow examination of dispersal and gene flow in this species across large tropical and subtropical oceanic ranges.",
author = "Wham, {Drew C.} and Margaux Carmichael and Reimer, {James D.} and LaJeunesse, {Todd C.}",
year = "2013",
month = "9",
day = "1",
doi = "10.1007/s12526-013-0150-y",
language = "English (US)",
volume = "43",
pages = "247--250",
journal = "Marine Biodiversity",
issn = "1867-1616",
publisher = "Springer Publishing Company",
number = "3",

}

Eight polymorphic microsatellite loci for the Indo-Pacific-wide zoanthid, Zoanthus sansibaricus. / Wham, Drew C.; Carmichael, Margaux; Reimer, James D.; LaJeunesse, Todd C.

In: Marine Biodiversity, Vol. 43, No. 3, 01.09.2013, p. 247-250.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Eight polymorphic microsatellite loci for the Indo-Pacific-wide zoanthid, Zoanthus sansibaricus

AU - Wham, Drew C.

AU - Carmichael, Margaux

AU - Reimer, James D.

AU - LaJeunesse, Todd C.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Next generation sequencing allows rapid development of genetic markers for investigating the ecology and evolution of non-model organisms. In the present study we employed 454 sequencing and high through-put screening to ultimately generate eight highly polymorphic microsatellites for Zoanthus sansibaricus, a common colonial anemone in the order Zoantharia that is widely distributed throughout the Indo-Pacific. Each locus was screened against cultured Symbiodinium as well as on individuals harboring distantly related symbiont species to discount contamination by DNA from their obligate dinoflagellate symbionts. The range of Z. sansibaricus extends across the tropical and subtropical Indian and Pacific oceans. Therefore, these population genetic markers will allow examination of dispersal and gene flow in this species across large tropical and subtropical oceanic ranges.

AB - Next generation sequencing allows rapid development of genetic markers for investigating the ecology and evolution of non-model organisms. In the present study we employed 454 sequencing and high through-put screening to ultimately generate eight highly polymorphic microsatellites for Zoanthus sansibaricus, a common colonial anemone in the order Zoantharia that is widely distributed throughout the Indo-Pacific. Each locus was screened against cultured Symbiodinium as well as on individuals harboring distantly related symbiont species to discount contamination by DNA from their obligate dinoflagellate symbionts. The range of Z. sansibaricus extends across the tropical and subtropical Indian and Pacific oceans. Therefore, these population genetic markers will allow examination of dispersal and gene flow in this species across large tropical and subtropical oceanic ranges.

UR - http://www.scopus.com/inward/record.url?scp=84881612464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881612464&partnerID=8YFLogxK

U2 - 10.1007/s12526-013-0150-y

DO - 10.1007/s12526-013-0150-y

M3 - Article

AN - SCOPUS:84881612464

VL - 43

SP - 247

EP - 250

JO - Marine Biodiversity

JF - Marine Biodiversity

SN - 1867-1616

IS - 3

ER -