Electrical molecular focusing for laser induced fluorescence based single DNA detection

Tza Huei Wang, Pak Kin Wong, Chih Ming Ho

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This paper details an electrokinetic focusing technique that significantly enhances the detection efficiency for laser induced fluorescence (LIF) based molecular sensing by concentrating fluorescence labeled molecules in a tiny probe region. 3-D electrodes were fabricated to generate wider and denser electric fields toward the probe region in the microchannel. The generated electric field is able to focus flowing DNA molecules to widths as narrow as 3 μm in a 120 μm wide channel. A single molecule detection technique is developed and the enhancement of detection efficiency by using the focusing method is demonstrated.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalProceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
DOIs
StatePublished - Jan 1 2002

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DNA
Fluorescence
Molecules
Lasers
Electric fields
Microchannels
Electrodes

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering
  • Control and Systems Engineering

Cite this

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Electrical molecular focusing for laser induced fluorescence based single DNA detection. / Wang, Tza Huei; Wong, Pak Kin; Ho, Chih Ming.

In: Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS), 01.01.2002, p. 15-18.

Research output: Contribution to journalArticle

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