Electrically assisted sampling across membranes with electrophoresis in nanometer inner diameter capillaries

Lori A. Woods, Parul U. Gandhi, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

A nondestructive method for sampling from ultrasmall environments has been developed utilizing electrophoresis in nanometer inner diameter capillaries and etched electrochemical detection. The desire to study increasingly smaller biological environments such as mammalian cells has led to the need for capillary electrophoresis techniques with subpicoliter volume sampling capabilities. This sampling technique involves the fabrication of a microinjector at the tip of a 770-nm-inner diameter capillary and the use of electroporation for insertion through the membrane. Separations of catecholamines sampled from the interior of intact liposomes have been achieved. A separation of a cytoplasmic sample taken from an intact mammalian cell has also been obtained.

Original languageEnglish (US)
Pages (from-to)1819-1823
Number of pages5
JournalAnalytical Chemistry
Volume77
Issue number6
DOIs
StatePublished - Mar 15 2005

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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