Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy

Jihong Zheng, Yanmeng Jiang, Aiqing Huang, Guoqiang Sun, Songlin Zhuang, Shizhuo Yin

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)

    Abstract

    A novel two channel multi-frequencies division multiplexed confocal fluorescence microscopy (FDMCF) system based on the holographic polymer dispersed liquid crystal (H-PDLC) optical chopper modulation is reported. The characteristic of unique FDMCF system is that within the FDMCF system, the multi-channel exciter laser beams are modulated with different carrying frequencies, after the fluorescence signals are collected, and through data analyzing process, the FDMCF system can realize the multiply points parallel detection synchronously with high temporal and spatial resolution. Besides, combined with the electrically controlled H-PDLC grating array working as the optical chopper to replace the mechanical chopper, it can improve and develop the FDMCF system from two channels to unlimited system. The paper has done the experiments to demonstrate the two-channel FDMCF system based on H-PDLC modulation and analysis the benefits from the electrically-controlled integrated electro-optical H-PDLC devices.

    Original languageEnglish (US)
    Title of host publicationPhotonic Fiber and Crystal Devices
    Subtitle of host publicationAdvances in Materials and Innovations in Device Applications IV
    DOIs
    StatePublished - Oct 18 2010
    EventPhotonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV - San Diego, CA, United States
    Duration: Aug 1 2010Aug 2 2010

    Publication series

    NameProceedings of SPIE - The International Society for Optical Engineering
    Volume7781
    ISSN (Print)0277-786X

    Other

    OtherPhotonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV
    CountryUnited States
    CitySan Diego, CA
    Period8/1/108/2/10

    Fingerprint

    Confocal Microscopy
    Fluorescence Microscopy
    Confocal microscopy
    Fluorescence microscopy
    Liquid crystal polymers
    Liquid Crystal
    division
    Division
    Polymers
    liquid crystals
    microscopy
    fluorescence
    polymers
    electric choppers
    Modulation
    modulation
    Laser beams
    temporal resolution
    Laser Beam
    Spatial Resolution

    All Science Journal Classification (ASJC) codes

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Computer Science Applications
    • Applied Mathematics
    • Electrical and Electronic Engineering

    Cite this

    Zheng, J., Jiang, Y., Huang, A., Sun, G., Zhuang, S., & Yin, S. (2010). Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy. In Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV [778115] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7781). https://doi.org/10.1117/12.862151
    Zheng, Jihong ; Jiang, Yanmeng ; Huang, Aiqing ; Sun, Guoqiang ; Zhuang, Songlin ; Yin, Shizhuo. / Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy. Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV. 2010. (Proceedings of SPIE - The International Society for Optical Engineering).
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    abstract = "A novel two channel multi-frequencies division multiplexed confocal fluorescence microscopy (FDMCF) system based on the holographic polymer dispersed liquid crystal (H-PDLC) optical chopper modulation is reported. The characteristic of unique FDMCF system is that within the FDMCF system, the multi-channel exciter laser beams are modulated with different carrying frequencies, after the fluorescence signals are collected, and through data analyzing process, the FDMCF system can realize the multiply points parallel detection synchronously with high temporal and spatial resolution. Besides, combined with the electrically controlled H-PDLC grating array working as the optical chopper to replace the mechanical chopper, it can improve and develop the FDMCF system from two channels to unlimited system. The paper has done the experiments to demonstrate the two-channel FDMCF system based on H-PDLC modulation and analysis the benefits from the electrically-controlled integrated electro-optical H-PDLC devices.",
    author = "Jihong Zheng and Yanmeng Jiang and Aiqing Huang and Guoqiang Sun and Songlin Zhuang and Shizhuo Yin",
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    Zheng, J, Jiang, Y, Huang, A, Sun, G, Zhuang, S & Yin, S 2010, Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy. in Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV., 778115, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7781, Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV, San Diego, CA, United States, 8/1/10. https://doi.org/10.1117/12.862151

    Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy. / Zheng, Jihong; Jiang, Yanmeng; Huang, Aiqing; Sun, Guoqiang; Zhuang, Songlin; Yin, Shizhuo.

    Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV. 2010. 778115 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7781).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

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    AU - Yin, Shizhuo

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    AB - A novel two channel multi-frequencies division multiplexed confocal fluorescence microscopy (FDMCF) system based on the holographic polymer dispersed liquid crystal (H-PDLC) optical chopper modulation is reported. The characteristic of unique FDMCF system is that within the FDMCF system, the multi-channel exciter laser beams are modulated with different carrying frequencies, after the fluorescence signals are collected, and through data analyzing process, the FDMCF system can realize the multiply points parallel detection synchronously with high temporal and spatial resolution. Besides, combined with the electrically controlled H-PDLC grating array working as the optical chopper to replace the mechanical chopper, it can improve and develop the FDMCF system from two channels to unlimited system. The paper has done the experiments to demonstrate the two-channel FDMCF system based on H-PDLC modulation and analysis the benefits from the electrically-controlled integrated electro-optical H-PDLC devices.

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    Zheng J, Jiang Y, Huang A, Sun G, Zhuang S, Yin S. Electrically controlled H-PDLC array modulated multi-frequencies division multiplexed fluorescence confocal microscopy. In Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications IV. 2010. 778115. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.862151